FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6107061
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG63450.078.513292
AGCGAAA63850.076.776581
CGAAAGC67450.073.3202743
GAGCGAA29700.062.2625161
GAGCAAA148350.053.1844061
GCAAAAG184300.042.1391533
CAAAAGC184900.041.9239734
AGCAAAA208850.036.907692
AAAAGCA248150.031.705685
AAAGCAG305000.031.3593166
AAGCAGG296500.030.1491227
GAAAGCA192450.026.7523574
AGCAGGG205600.020.4171588
GTATAGG24900.020.3864541
TACGACG7200.020.138817
ACGACGC7300.019.8630968
CGACGCT7450.019.4631719
CACGAGC17250.018.072393145
TATACCG3352.8573648E-517.3167675
CTTTCGC14100.016.9712142
Produced by FastQC (version 0.11.5)