FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7671657
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG137810.17963524698771074No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA111420.14523589884167137No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA111020.1447144990971312No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC94420.12307640969871306No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC92520.12059976091214715No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT89140.11619393307078249No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA88630.11552914839649375No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG82270.10723889245830465No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG82210.10716068249662361No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT80980.10555737828216252No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT80470.10489259360787376No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA77060.10044766078566861No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG94150.083.7204062
GAGCGAA35450.079.164081
AGCGAAA99250.078.909031
CGAAAGC100350.078.476613
GAGCAAA240000.064.17661
CAAAAGC295200.051.7086874
GCAAAAG332100.045.8977743
AAGCAGG390600.044.604197
AGCAAAA353600.043.2500342
AAAAGCA368600.042.0422025
AAAGCAG444300.040.852976
CCGCACG5600.040.13333145
GAAAGCA201150.039.8715254
GGTCGTT32700.038.8011367
AGTAGAA104350.034.6770021
CAATTCG62800.033.827349
TAGAAAC113000.033.4976352
AGCAGGT170000.033.394338
GTAGAAA124550.031.9058441
GCAGGTC79400.031.5034719
Produced by FastQC (version 0.11.5)