FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6999373
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG84650.090.622832
CGAAAGC91400.083.694633
AGCGAAA92350.082.362571
GAGCGAA26750.077.79461
GAGCAAA138150.048.9690971
GAAAGCA197500.039.5035864
CAAAAGC205200.033.144914
AAGCAGG324650.032.6085856
GCAAAAG207850.032.3734743
AAAGCAG359350.031.8407885
AGCAAAA222200.030.1513562
CTCGATA25800.029.2254719
TAGACCG2802.6375346E-1028.4858465
AGCAGGG212800.026.5746637
AAAAGCA270100.025.3957465
CCTCGAT33300.022.4254388
CAATTCG6050.021.5707389
GACCGTA4551.546141E-1020.714617
TATATCG6400.020.3932765
GGCCTCG37750.019.9761076
Produced by FastQC (version 0.11.5)