FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4404863
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA143300.3253222631441659No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA141510.3212585726275709No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG126650.2875231306853357No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT116740.2650252686632933No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC113890.2585551468910611No Hit
GAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGAACTAC102050.2316757638092263No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT93830.21301457048720926No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC90400.20522772217887367No Hit
TTTTTAAACTATTCAGCATTAATTGATGGCCATCCGAATTCTTTTGGTCG82430.1871340833982805No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC78040.17716782565087721No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA74910.1700620427922503No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA69730.15830231269394757No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA68710.15598669016493816No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA67710.15371647199924265No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC67710.15371647199924265No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC66360.15065167747555372No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT63830.14490802551634407No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA61090.13868762774233842No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG59750.13564553540030644No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT58780.1334434237795818No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT55720.12649655619255354No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC55220.1253614471097058No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC54400.12349986821383549No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT52940.12018534969192003No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG52760.11977671042209484No Hit
GTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA51360.11659840499012115No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG50680.1150546566374482No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA48770.11071853994096979No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG48770.11071853994096979No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTGT48350.10976504831137768No Hit
CCTATAATGAGAAACCCTCTCAAGACGGCGGACTCCACTCCGGATGTGCT46860.10638242324449138No Hit
GGATTATAGGTAAGGAAGACAGAAGGTACGGACCAGCATTAAGCATCAAT46230.10495218580010321No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT46130.10472516398353365No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG44310.10059336692196783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA27750.0107.1435241
AGTAGAA39300.076.208351
GCAAAAG171050.073.722813
CAAAAGC182950.069.640954
CGAGTCG1600.067.96776145
AGCGAAA48600.063.5650561
CGAAAGC49900.063.2141153
GAGCAAA208500.061.561941
AGGCTCG5550.061.395504145
GCAGGGT64050.061.3517049
AAGCAGG264850.058.3611537
AGCAGGC26550.058.1643148
GCGAACG502.0090007E-458.011673
AGCAAAA222250.057.2932132
GCGAAAG55750.056.5802462
AGCAGGG102550.056.5582358
CAGTACG23100.056.180759
TAAACTA30600.049.2909625
AGGGTGT32200.049.0937276
AAAAGCA261950.048.9151655
Produced by FastQC (version 0.11.5)