FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6157095
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGAAGTGACTGAGTCGGAGATAGCACAAGGTATTGACCAACAGATA64610.1049358504294639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC102250.076.5880053
AGCGAAA105350.073.5084151
GCGAAAG152050.051.5023542
GAGCAAA149600.045.755231
GCAAAAG167450.040.4447443
GAAAGCA227500.035.410584
GAGCGAA67500.035.2346881
AGCAAAA195450.034.9095082
CAAAAGC198450.034.1268424
AAGCAGG298750.033.882966
AAAGCAG407300.028.98285
AGCAGGG174600.026.07717
GTATAGA55900.025.2943271
AAAAGCA293000.023.906165
TCTATAC50150.023.4231033
TAGGACC11650.022.4066494
GTGCGTT18200.021.1132689
TACTATC48800.019.6109247
GGTGCGT25250.018.6637738
GCAGGGG81100.018.5053358
Produced by FastQC (version 0.11.5)