FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACATATAAGAACATTGGCCTTGAAATTTGGCCTATGGCACTTCTTA25.263157894736842No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT25.263157894736842No Hit
CATTTGGCCTGGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCAC12.631578947368421No Hit
CCAAACGGTCTTATGAACAGATGGAAACTGATGGGGATCGCCAGAATGCA12.631578947368421No Hit
GAAGTAACCCCTAGGAGCAATTAGATTCCCTGTGCTGTTAATCAAAAGTA12.631578947368421No Hit
TAAAAATGCAACTGCTAGCTTCATTTACAATGGGAGGCTTGTAGATAGTG12.631578947368421No Hit
GGTTGGTGGTGAGAGGAAGACTTTGCTGGACTGGGGCCCCATGAAGTGGA12.631578947368421No Hit
CGCTCACTGGGCACGGTGAGCGTGAACACAAACCCCAAAATCCCCTTAGT12.631578947368421No Hit
GAATAAATCAGAAGCATGGCCCATTGGGGAGTCCCCCAAGGGAGTGGAAG12.631578947368421No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT12.631578947368421No Hit
CTTTTCTACAATTTGCTTTCCAACATGGGTGGCTGCTTTGATGTCTAGAC12.631578947368421No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAACCC12.631578947368421No Hit
CAGGGAATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAGTGGGAAA12.631578947368421No Hit
TTGTATCCTGCAAATTATTTCATTATTGTTTTATGAATTACATTGCCAGA12.631578947368421No Hit
CTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAA12.631578947368421No Hit
AAATGGTACTCTCTGCTTTTGATGAAAGAAGGAATAAATACCTGGAAGAA12.631578947368421No Hit
CTCCATTCCCATTGAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCT12.631578947368421No Hit
GTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTTGTTCA12.631578947368421No Hit
CTTAAAAGAGGCCCTTCTACGGAAGGAGTACCTGAGTCTATGAGGGAAGA12.631578947368421No Hit
CCATCATTCTTATGATTTCTGCCCTCATGTCTGAAGTTCTTCCCTCCGTA12.631578947368421No Hit
CGCTGAGATCGAAGACCTCATATTTTTAGCAAGATCTGCATTGATATTGA12.631578947368421No Hit
GTGCACTTGCCAGTTGCATGGGCCTCATATACAATAGGATGGGGGCTGTA12.631578947368421No Hit
CAACCCAATTGATGGACCACTACCTGAGGATAATGAACCAAGTGGATATG12.631578947368421No Hit
ACACCATATAAGTGAGAAGTCAGGCTTTAAGCCAGGGAGAGACCTGCCCA12.631578947368421No Hit
GAAAAAAAGACGATCAAGAATCCACAATATCAAGTGCAAGATCCCAATGA12.631578947368421No Hit
TGCAGATACTGTTTACTACTGTCCAGGCCATTGTTCTGTCTCACCCCTCG12.631578947368421No Hit
CCCTAAGGGGAAGAGGCAATACTCTCGGTCTAGACATCAAAGCAGCCACC12.631578947368421No Hit
TCATAAGGGTAACAGTTGCTGTAGGCTTTGCTGCGTTCAACAAAAAGGTC12.631578947368421No Hit
GAGGCCCTCTGTTGATTAGTGTTTCCTCCACTCCTGGTCCTTATGGCCCA12.631578947368421No Hit
GAGGCAAGAGAGTCACTTGAGTATGGGAGGTGGGGGCTGCAGTGAGCTGA12.631578947368421No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAATAGTAGCAC12.631578947368421No Hit
GATGATGGTAATACCTTAAAGGAAGAGAGGTCCAACAAAAAGCTGGAAGC12.631578947368421No Hit
CTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAG12.631578947368421No Hit
ATTCTAAACACAAGCCAAAGGGGAATTCTTGAGGATGAACAGATGTACCA12.631578947368421No Hit
TCTGTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGG12.631578947368421No Hit
AGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAA12.631578947368421No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)