FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4875604
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA91210.1870742578765626No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC57780.11850839403692343No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT57370.1176674725839096No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG54120.11100163179782443No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC52710.10810968241063057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA30150.088.74861
AGCGAAA52250.065.644351
GAGCAAA169500.062.7599871
CGAAAGC58600.060.1365933
GCAAAAG175800.060.012863
GCGAAAG62750.055.812772
CAAAAGC190800.055.4088754
AGCAAAA190400.054.9921152
AAGCAGG262300.048.7837647
AAAAGCA236000.044.8581165
AAAGCAG321450.040.6711966
AGCAGGG137700.039.0137448
AGGCTCG5850.034.70084145
AGTAGAA52100.033.8213541
CAGTACG7250.032.999999
CGCGAAA1204.055709E-430.2142071
TAGAAAC69700.029.3370532
GCAGGGT78550.029.0738289
GCAGTCG3403.6379788E-1227.7248426
GCAGGTA80450.027.5761229
Produced by FastQC (version 0.11.5)