FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8416570
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG161660.19207349312130714No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA124290.14767298317485625No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA115430.13714612959911224No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT95190.11309832865407166No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT86410.10266652567494834No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA40700.089.798671
GAGCAAA228950.072.943581
AGCGAAA75600.072.611911
GCGAAAG79300.069.9534452
CGAAAGC83850.065.986523
AGCAAAA325600.050.577062
CAAAAGC338450.048.9796264
AAGCAGG403700.048.5269437
GCAAAAG366650.045.2718123
AAAGCAG482700.041.5843856
AAAAGCA420250.039.722735
GCGAAGC8100.039.39143
AGCAGGG240050.039.0528878
GTAGAAA109750.036.4727361
TAGAAAC117800.034.1639862
CAATTCG21850.033.8466269
CGTGTAG10500.032.4601145
TCGTGTA10750.032.3792344
GCAGGTA72450.030.122769
AGTAGAA100750.027.998731
Produced by FastQC (version 0.11.5)