FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2653335
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA21800.090.8201751
GAGCAAA78100.081.994711
AGCGAAA41900.075.984791
GCGAAAG49400.066.207842
CAAAAGC102850.062.2633674
CGAAAGC52900.062.1038783
AGCAAAA102350.061.785772
GCAAAAG110450.057.6507533
AAAAGCA125750.051.213115
AAAGCAG179450.045.9508136
AAGCAGG184600.043.8707357
AGTAGAA45050.031.2308041
AGCAGGC55550.031.1931388
GAAAGCA108700.030.957414
TAGAAAC47100.030.0254763
GCGAAGC5050.028.7220083
AGCAGGG102000.027.578898
ATCGTGT8300.027.0869333
AGCGTCA4300.026.9853293
TATCGTG8950.026.7393422
Produced by FastQC (version 0.11.5)