FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7966943
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA266610.33464529619453787No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC141180.17720724247681952No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT129140.16209479595875106No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA123110.15452602083383804No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT119000.14936720395765352No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG118350.1485513326755319No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG112580.1413089060634675No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT110010.13808307653261737No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC107940.1354848402957069No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG100310.12590776663018677No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC99730.1251797584092167No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT99310.12465258004230731No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG95700.12012135646006254No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT94910.11912975905563777No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG93780.11771139821133401No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA91940.11540185488963584No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA83180.10440642037981193No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG81930.10283743714496263No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG80150.10060320501853721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA44850.084.396051
GCAAAAG227150.070.164613
AGGCTCG10150.065.71384145
AGTAGAA49650.064.699151
AAGCAGG328000.060.0548557
CAAAAGC281150.057.56514
CGAAAGC66450.056.633784
AGCGAAA68150.054.582662
GAGCAAA306750.052.8804931
AGCAGGT160600.052.456068
AGCAAAA332100.048.580762
GCGAAAG82700.046.3824163
GCAGGGG68850.044.2271089
CGTGTAG10550.043.987925
AGCAGGG163900.043.835888
TATCGTG11600.042.506382
AAAGCAG467800.042.161366
GTCGGCT4850.038.8664479
AAAAGCA427950.038.1915055
CGTCAGA6050.037.154342
Produced by FastQC (version 0.11.5)