FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7161038
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCCCAAGCAGAAAATGGAAGGACCTCTTTGCATCAGAATGGACCAGGC475320.6637585221583798No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC257620.359752315236981No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT213260.2978059884614493No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA207660.289985893106558No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG180920.25264493778695213No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA179210.2502570158125121No Hit
GTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA173750.24263242284149308No Hit
GAAAATGGAAGGACCTCTTTGCATCAGAATGGACCAGGCAATCATGGAGA171580.23960213589147272No Hit
GTACAAATTGCTTCAAATGAGAACATGGATAATATGGGATCGAGCACTCT164980.23038559493749367No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT154760.2161139209148171No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC153260.2140192525161855No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT141370.1974155143430324No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT136690.19088014893930183No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC128380.17927568601088278No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT124610.17401108610232205No Hit
TCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTC122760.17142766174400972No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA122580.17117630153617394No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA122150.1705758299285662No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC117060.1634679218292097No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT116330.16244851654187564No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGC116070.16208544068611283No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG112060.156485693833771No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA107320.14986654169409516No Hit
GGAGTGGAGGAAACACTAATCAACAGAGGGCCTCCGCAGGCCAAACCAGT101260.14140408136362356No Hit
TCTCATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGA100820.14078964530002494No Hit
TTGTCATACTCTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACT100180.13989592011660879No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG100150.13985402674863617No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT100000.139644559908773No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA96890.13530161409561017No Hit
CATTACTGCTTCTCCAAGCGAATCTCTGTAGATTTTTAGAGACTCGAACT91380.12760719884463675No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG89930.12558235272595958No Hit
GTTCTTATCTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAA89620.12514945459024238No Hit
CTTATCTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGT88750.12393454691903603No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT87680.12244035012801217No Hit
GGATCAAGTGAACAGGCAGCGGAGGCCATGGAGATTGCTAGTCAGGCCAG85250.11904698732222899No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA82820.11565362451644581No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC82320.11495540171690194No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG81230.11343327601389631No Hit
TTTTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAAC81190.1133774181899328No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT80210.11200890150282683No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG79640.11121292751134682No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT79320.11076606491963875No Hit
GTTTTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATCTCTTCAAA77610.10837814294519872No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT76040.10618572335463099No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA73930.10323922314055588No Hit
ATATACAGGAGAGTAGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA72050.10061390541427095No Hit
TCTTATCTCTTCAAACTTTTGACCTAGCTGTTCTCGCCATTTCCCGTTTC71970.10050218976634392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC63750.078.371463
AGCGAAA65600.074.947461
GAGCGAA26000.069.447561
GCGAAAG75150.066.481842
GCAAAAG109850.058.882153
TAGAAAC87700.056.2240522
AGTAGAA54400.055.0531461
CGAGTCG4450.052.13471145
AGGGTGT79800.048.888236
GCAGGGT68700.046.4349569
CAGTACG14300.045.6305359
CCCAAGC176050.043.8252564
GCCCAAG178200.043.337193
ACAAGGG112200.043.172033
CAAAAGC152700.042.833784
CAAGGGT118050.040.97124
TGCCCAA188950.040.90942
AAGGGTG101250.039.677085
CATACTC69350.038.2695583
ATGCCCA202650.038.073781
Produced by FastQC (version 0.11.5)