FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAGACCCTATTGATGAATGAGTTAGGTGTTCCTTTTCATCTGGGGAC22.7027027027027026No Hit
CTCTTGTGTGCTGGATTCTCGTTAGGTCTGATTAAGCTGTATATTTGGCT22.7027027027027026No Hit
AGCCACTCATGTTGGAAAGCAAATTGTAGAAAAGATTCTGAAAGAAGAAT22.7027027027027026No Hit
GAATTAAGGTCACTTTCAAGCTGGATACAGAATGAGTTCAACAAGGCCTG11.3513513513513513No Hit
TAAGTGGGTCAACCAATTCAATCTACTAAAGAAACTGTTTTTAGATCTCC11.3513513513513513No Hit
CGGGAATACAAGCAGGAGTGGATAGATTCTACAGGACCTGCAAGTTAGTG11.3513513513513513No Hit
CACCAGCTGACTCTTGTGTGCTGGATTCTCGTTAGGTCTGATTAAGCTGT11.3513513513513513No Hit
AACTGCATTCTGCTTTGCTTTGGTGGAGCAGCTGCAAAGGGGAGAAGCTT11.3513513513513513No Hit
CCCTCTAATTTGCATCCCGGGTGTTGCAATAGCCCTTCTTTTTAATTTGC11.3513513513513513No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATGTTC11.3513513513513513No Hit
TTCCTCAATAGTCATGTCAGTTATGTATCGCGAAGCAGGTGTGGAGACCA11.3513513513513513No Hit
AGTAATGACTGATGGGAGTGCTTCAGGAAAAGCTGATACTAAAATATTAT11.3513513513513513No Hit
GGAGCAGTTTTCTTGACTCCGCTGAAAATCATTCCAATTGTGGTGATGCA11.3513513513513513No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG11.3513513513513513No Hit
GTTGTTTAGTGCTTCATCTCTGTATACATCGTGGTCATAAGTTCCATTTC11.3513513513513513No Hit
ATGCAGATCTTGCTAAAAATATGAGGTCTTCGATCTCAGCGTTTCCTGGG11.3513513513513513No Hit
TTTTTAAACTATTCAGCATTAATTGATGGCCATCCGAATTCTTTTGGTCG11.3513513513513513No Hit
ATGCTAATGCCCAAGCAGAAAATGGAAGGACCTCTTTGCATCAGAATGGA11.3513513513513513No Hit
CCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTTGACATCCTCAA11.3513513513513513No Hit
ACTGGCAAGTGCACCAGCAGAATAACTGAGAGCTATTTCTTTGGCCCCAT11.3513513513513513No Hit
TTGTACAACAAAATGGAATTTGAACCATTTCAATCTTTAGTCCCCAAGGC11.3513513513513513No Hit
CCTCATATTTTTAGCAAGATCTGCATTGATATTGAGAGGATCAGTTGCTC11.3513513513513513No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT11.3513513513513513No Hit
CTTATGATTTCTGCCCTCATGTCTGAAGTTCTTCCCTCCGTATTTCCAGT11.3513513513513513No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA11.3513513513513513No Hit
CTCCTGGTCCTTATGGCCCAGTACCCGATTCTCAGTTCAACAGTGCTCGA11.3513513513513513No Hit
GACTGCAGCGTGGACGCTTTGTCCAAAATGCCCTCAATGGGAATGGAGAC11.3513513513513513No Hit
TCCTGATTCATTAGCTGTTAATCCGTTCGATCTAAAAACTTCTATGGTGT11.3513513513513513No Hit
GTCAAGTTCTACCACTATTGATTCGCCTTGTTCATTGATGAAATGGAAAT11.3513513513513513No Hit
GTATATGGGCCCCCCAGTTTTCTTGGGATCTTTCCCCGCGCTGGGGTGTT11.3513513513513513No Hit
GCTTCAACCCGATGATTGTCGAACTTGCAGAAAAAGCAATGAAAGAGTAT11.3513513513513513No Hit
CTGTAGAATCTATCCACTCCTGCTTGTATTCCCTCATGATTTGGTGCATT11.3513513513513513No Hit
AGATGCTTATTCTGCTAGGGATATTCCTTACTCTGGGTCTAGATCCGATA11.3513513513513513No Hit
TTATTGAGCTTTTCCCACTTCGTATTTTGAAGTAACCCCTAGGAGCAATT11.3513513513513513No Hit
CTTCTCTCCTTGTCACCCCAATTTCGATGAATCTGTTCTCCTTGTAATCA11.3513513513513513No Hit
GGCCCAGCATTCTGCTGTTCCTTTCGCTATTCTTCCCGCGTAGACTCAGG11.3513513513513513No Hit
GCCTTAAAAGAGGCCCTTCTACGGAAGGAGTACCTGAGTCTATGAGGGAA11.3513513513513513No Hit
GTCTAAGAATGTCCACCATCCTTGTTCCGCCAATTTGTGTGCTGTGGCAC11.3513513513513513No Hit
ATGTCACATTGCGGCTTTGACCAATTTCTGTATTCTGCTAGTTTGGGGCA11.3513513513513513No Hit
AGCTGGTTCAGAGTTCCTCAACAGGTGAAATATGCGACAGTCCTCATCAG11.3513513513513513No Hit
GGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATATGAGG11.3513513513513513No Hit
CAGTTTGTTGGCTTCTTTTGGTAGAGACTGTGATTCTTCCTGATGCTTGA11.3513513513513513No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC11.3513513513513513No Hit
GAGCACTTCCTGACAATGTGCTAGTATGAACGATTTTTCCCTCCTCAATG11.3513513513513513No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCATTATTTT11.3513513513513513No Hit
TTCTTTTTCAATCTGATGGAATTTCTCGTTGGTTTTCCCGATCAATCTAT11.3513513513513513No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT11.3513513513513513No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA11.3513513513513513No Hit
CTCTTGTGTGCTGGATTCTCGTTAGGTCGGATTAAGCTGTATATTTGGCT11.3513513513513513No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG11.3513513513513513No Hit
TTCTGTATCCAGCTTGAAAGTGACCTTAATTCAGGTTCGTCACTATCATA11.3513513513513513No Hit
TAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAATT11.3513513513513513No Hit
ACCGGTTTCAGATCAAGGGAGTTGAGCTGAAGTCAGGGTACAAAGATTGG11.3513513513513513No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT11.3513513513513513No Hit
GACTTGAATGGAATGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGA11.3513513513513513No Hit
CTTCATTACTCATATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCG11.3513513513513513No Hit
GGGGGGTGGTGCGATGCTCAGGATGTTTCTGCACCACTCAGGTTGATTCT11.3513513513513513No Hit
GTTCCCAACACGTTCCCTGAGTCAAATGTAAAACTTCAATGTATATACTG11.3513513513513513No Hit
GATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGAAATGTA11.3513513513513513No Hit
GTATATGGGCCCCCCAGTTTTGTTGGGATCTTTCCCCGCGCTGGGGTGTT11.3513513513513513No Hit
TTGCGGAAGCTTATACAGGCATGAGATTGATGTCCGCCCCATCAGGCCAT11.3513513513513513No Hit
CATTCTATGTCTGGTTTTCGCTCAAAAAATTCCTGGAAATGACAACAGCA11.3513513513513513No Hit
AGATAGTGTATCTGACCAACACCACCATAGAGAAGGAAATATGCCCCAAA11.3513513513513513No Hit
AGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAA11.3513513513513513No Hit
TCTCATGTTTTATTAATGGATTGGTTGTTGCCACCATCTGCCTATGAGAC11.3513513513513513No Hit
GCTTGAGCATACAGGAAGATTTGGTCTTTGTCCGTACCCGGGTGGTGAAC11.3513513513513513No Hit
ATGTTGTTCTTTATCACTGTTACTCCAATGCTCATATCAGCTGACTCGTT11.3513513513513513No Hit
CCATTCTGCTGCATTTGAAGATTTAAGATTGTTAAGCTTCATCAGAGGGA11.3513513513513513No Hit
TCAAAAGTATGTCTCCCGGTTTTACTATTGTCCAATAGATGCTTATTCTG11.3513513513513513No Hit
TCTTCCTGTATGCTCAAGCATCAGGAAGAATCACAGTCTCTACCAAAAGA11.3513513513513513No Hit
CTTCAAGGAAGGCCATAGCCTCCAGGACACAGTCTGTTTGTGCATATCCA11.3513513513513513No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTT50.0145.08
CCAACAC50.0145.05
TCCCAAC50.0145.03
CACGTTC50.0145.09
GTTCCCA50.0145.01
CAACACG50.0145.06
TTCCCAA50.0145.02
CCCAACA50.0145.04
GGAGTTG50.0145.0145
AACACGT50.0145.07