FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7215116
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA235400.086.0700761
GAGCGAA62950.067.96551
GCGAAAG100700.064.591322
GCAAAAG315900.063.492993
AGCGAAA100900.063.3883821
AGCAAAA316100.063.313422
CAAAAGC323950.062.0047574
AAAAGCA378100.053.4706345
CGAAAGC121750.053.4252623
AAAGCAG497600.048.965276
AAGCAGG460950.048.8695567
AGCAGGG237700.038.6149528
AGCAGGC144050.031.7086878
CGATTAC31700.026.7644841
GCAGGGG126150.026.55279
GAAAGCA265250.025.479084
CGGTCAC15300.025.1196585
CCCTGTA67200.024.4912289
AGTAGAA93800.024.0430641
TACGGGC15700.024.0174524
Produced by FastQC (version 0.11.5)