FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10027617
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA287200.078.093471
GCGAAAG113150.069.14422
AGCGAAA119850.064.375831
CGAAAGC139950.056.2679373
AGCAAAA394700.055.8280532
GCAAAAG401650.054.900053
CAAAAGC414900.053.1992234
GAGCGAA79100.050.7869951
AAAAGCA493550.044.9860765
AAGCAGG571200.042.8511057
AAAGCAG627500.041.4035076
AGCAGGG294000.032.7735338
AGCAGGC181100.027.3030728
GAAAGCA318250.025.5411994
AGTAGAA127550.023.5932041
GCTAGCC34850.023.5114693
AGCAGGT204400.021.8142288
GCAGGGG150000.021.2670389
AACAAGG219700.021.056182
GTAGAAA167650.021.0209311
Produced by FastQC (version 0.11.5)