FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5618801
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTGTGTAGTTAAAACTAATACTCGGTGTCATCATATGT168540.29995723286872056No Hit
ATTCTAAAGTGAATAATTATAGCATACAATGGATACACACTCAAGATGCC120180.21388904857103855No Hit
ATATAATGGTACAAACGGGACTTAATTTCCGTAACGATGTGAATACCACA116760.20780234074849777No Hit
CTCCAACAGACGCGGTTGAAGCTCTTCCAATCCTACACATCGATGAGCCA90870.16172489468838636No Hit
CATTTCACACCATCGATTTCTTTTACGATAACTTCTGGACGAGATACTTG86610.15414320599715137No Hit
ATCCTGAGATATGTCTATTTGGAGTAATCACTTCTCTATTGGTTGCAGCA81210.14453261469840273No Hit
ATTTTACCTTGACTCTTTCAGCAAATACGAATAATGTACTGACTACCAAA76400.13597206948599888No Hit
TAATTATAGCATACAATGGATACACACTCAAGATGCCAAGGCAAATCCTT75780.1348686312257722No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG74040.13177188514061985No Hit
CATCTATACAAAGGGTAGTTGGGCTGTGAGGCCTGAGATTAATTATAGAA73830.13139813992344632No Hit
GTACATTATTCGTATTTGCTGAAAGAGTCAAGGTAAAATAGCGTAAAACA72720.12942262948981464No Hit
CCATTATATTTCTCAATAGCCCATCTTCTGCTCCGCCTTTTCGTTTAAAT71490.12723355036065523No Hit
ATTATCAACCGATTGGCGAACACTTTGATTTAAAAATACTTACTGACATC70800.1260055303613707No Hit
CTTTAGAATAGTCATCAAGTCCTTTTATCCCTCTTAAAGTGGAAGAAATT66670.1186552077569574No Hit
GAGCAAAAGCAGGGATTCTTATTCCAAGTTTTGGGGAGAGAGAGACTGAC66070.11758736427931868No Hit
GTAAATATGCTGATTATTCAGCGAGTTGGTGTAGAAATTACTGCTATTGA65800.11710683471438123No Hit
ATACAAAGGGTAGTTGGGCTGTGAGGCCTGAGATTAATTATAGAAAAAAC65070.11580762514992078No Hit
TTTCTACACCAACTCGCTGAATAATCAGCATATTTACAATGGAAATAATC64110.1140990755856988No Hit
ATTCTATCTATTTAAACGAAAAGGCGGAGCAGAAGATGGGCTATTGAGAA63700.11336938254264567No Hit
TCTCAATACTCAACAAAACTCGAGTATAAGTTTCACAAAGCGATTCCTCA62050.11043281297913915No Hit
TCTCTATTGGTTGCAGCATCATAACTTTTGGTGATAGTTTTGGTAGTCAG61100.10874206080621115No Hit
AATCAATTCTATCTATTTAAACGAAAAGGCGGAGCAGAAGATGGGCTATT59930.10665976602481561No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA56830.10114257472368215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA34850.0106.949271
GAGCAAA156450.099.0481641
AGCAAAA228550.067.511382
AAGCAGG287300.064.1509867
CAAAAGC245350.063.3938564
GCAAAAG252600.061.717883
GACGCGG32100.059.180029
AAAAGCA265950.058.783925
AGCAGGT129950.057.3566938
AAAGCAG336900.057.0802886
AGACGCG34950.054.5601848
AGCGAAA79150.051.945711
CGAAAGC83800.050.2733733
GCGAAAG83500.050.104412
AGCAGGG171200.043.7485668
GCAGGGT50000.042.634019
CGTTCCG3900.039.0410928
CCCGCGT3800.036.254062
GCAGGGG52600.035.977729
GGTAACG9800.035.5167051
Produced by FastQC (version 0.11.5)