FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7816676
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCAAAGGTATGGTTCATGATACCGTAACCACGAGTCATTGACAAGAAC92860.11879729951708373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA39350.096.7476351
AGCGAAA103100.076.8050461
GCGAAAG106600.075.026792
GAGCAAA231650.073.0311661
CGAAAGC110750.072.346343
CAAAAGC295650.056.7523044
AGCAAAA299900.055.851332
GCAAAAG307400.054.6301883
AAAAGCA369900.045.792265
AAGCAGG457600.042.9672747
AAAGCAG480350.042.7804766
GCGCGAA1903.6379788E-1241.982171
GAAAGCA261600.031.3212224
CGCGAAA2401.5825208E-930.212512
AGCAGGG278850.029.7693238
GACGCAG38750.028.4388229
TCGCTCC23100.025.7398175
AGCAGGC156550.025.1930038
TTCGCTC23700.024.7819064
CAGACGC45350.024.2996717
Produced by FastQC (version 0.11.5)