FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAAACGAAAAGGCGGAGCAGAAGATGGGCTATTGAGAAATATAATGG23.1746031746031744No Hit
ATTTTACCTTGACTCTTTCAGCAAATACGAATAATGTACTGACTACCAAA23.1746031746031744No Hit
GCCTTGTGTGGACGTCAACGCCACCAGGAAGGACCATCAGGCCGTGGGCG23.1746031746031744No Hit
GCAAAAGCAGGACAGGCAAGGCTGGTAAGAAGGAACCAGTTAAGAGGCTG23.1746031746031744No Hit
GGTGAATATTGCCGTTAATCAATTCTATCTATTTAAACGAAAAGGCGGAG11.5873015873015872No Hit
CTCATTACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGAGACTCGAA11.5873015873015872No Hit
ATAATAAAGATCATTTGGAAATTTGTATGTAATAGCCAAATGATCTTTAT11.5873015873015872No Hit
CTCTAGAGGAGTAAAGGCATTTATTTCTTCTTCTCTACTGATGTACCACA11.5873015873015872No Hit
GCCTTAAGGAAAAGGAACAATTGGGGCCAAAGTGGAGTAGAATAAAAATG11.5873015873015872No Hit
ACAGACCCTTGGTATTCTTCTGGAGTGTCGATTTGAACACGCTCAAATGG11.5873015873015872No Hit
AGGCAGTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGG11.5873015873015872No Hit
ATATTAGTGTTTTTGGAAGTATCAAAAACGCAGATGGATAATTCATAGTT11.5873015873015872No Hit
GAAATAAACGCCATCGGCATGCGCAAACTCAAAGCCTTGAATATCTCCCT11.5873015873015872No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11.5873015873015872No Hit
CTTTTCCCCTTGCTCATGACAATCAGAGAAACAGCCAGACCTGTTGACAT11.5873015873015872No Hit
TTGGTAGATTGAGGAATCGCTTTGTGAAACTTATACTCGAGTTTTGTTGA11.5873015873015872No Hit
GATTGACGTTTTATTTCATCCACTAAGTCCCATTTTTGCTGTAGATTAGC11.5873015873015872No Hit
TCTGTCGTGAAGTTTTGAGAATAATTAGCAGACCCAGTAATTGTAATTGG11.5873015873015872No Hit
ATTCGTATTTGCTGAAAGAGTCAAGGTAAAATAGCGTAAAACATTGGTAT11.5873015873015872No Hit
TTGCACCTGATATTGTGGATTACTGATCGTCTTTTTTTCAAATGTATTTA11.5873015873015872No Hit
GGTATGGAAGATATCTTTGTCGGTGAAACCATCACTCCAACAGACGCGGT11.5873015873015872No Hit
ACTCACCATTTGCTGGTAAAGAAGGTAAATGGGTAACTTCTCGTAAGGTA11.5873015873015872No Hit
GAGCAAGGACGTGATCATTAAAAATTAAGTATCATTTAATTTTTTCTGCC11.5873015873015872No Hit
AGTCAAGGTAAAATAGCGTAAAACATTGGTATTGGTAGAGATATTTATGG11.5873015873015872No Hit
ATGACAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCCGCAAGCGATT11.5873015873015872No Hit
TAAATATCTCTACCAATACCAATGTTTTACGCTATTTTACCTTGACTCTT11.5873015873015872No Hit
AGTGAGGTTCCATGAGGTGGCAAGATGAAAAAGCTCAGTACCTGCTCTGG11.5873015873015872No Hit
GAGCAAAAGCAGGCAGAGGGTGGAGGCAGAAGGAACAGTGGAAAAGCTGG11.5873015873015872No Hit
CTTCAACACTATCTCAATATGTTATGCAATCATTCATATAGCCTAAGAGA11.5873015873015872No Hit
GGGGCATCATATGTCGTATAGCCTTCACAAAATATTTATTTCCAAAATTT11.5873015873015872No Hit
GTTTTATAGGTGTTGGGGTTTGAGAGATTAGTCGGACACGATCGTCAGGG11.5873015873015872No Hit
GAAGGAGGGAAGACAGAAGGACTAAATGGCTGAATAAGTGAATAAATGAA11.5873015873015872No Hit
ACCCTCGAGGAACTGTCACGAGACTGGTTCATGCTCATGCCTAGGCAAAA11.5873015873015872No Hit
GTACCTACTTCGCGCTACCTTTCTGACATGACCCTCGAGGAACTGTCACG11.5873015873015872No Hit
GTCATGGTGAATGCACGGTCAGCGTGAGCGTGTGCATTTACCCAGCCTCC11.5873015873015872No Hit
CTACTGGTTCAATTCTTTCCATTTTCTTACTCCTTTTCTCCGACTTCTTG11.5873015873015872No Hit
AGTATAGCCTTTGTCCGTTACTGCCCTGATGATGATACCTGCAAATATCA11.5873015873015872No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC11.5873015873015872No Hit
CAGGATCCCTGATGCCTATGACAGTGGCTGCCACATACACACAAGGGTGT11.5873015873015872No Hit
ATATAAGGTCTTCTATATTCTTATTCCTGCCTACCTTCCAAGCACATCTC11.5873015873015872No Hit
AAAGCAGGTAATGATCCAAAATGGAAGACTTTGTGCGACAATTCTTAAAT11.5873015873015872No Hit
GCATTATTGGTAATACCCAAGAGGTGGAAACAACTGAAGTGTCGATTGAT11.5873015873015872No Hit
TTTTCATCTCGTCCCACATTTCTTTTTTGAAAAGAAAATGACTTGTGGTA11.5873015873015872No Hit
GCCCGTACAGAGGAGCAAGGTGGAGTAATCGTCTCCGAAAAAACAAAAAA11.5873015873015872No Hit
ATTTTATAACGTAGGAAAAAAAATCATTATGTGAATATGATCCAAGCAAT11.5873015873015872No Hit
GTACATACAACCGCATCTGGACGTACTGCTTTCGCATCGTGCGGAGTTGT11.5873015873015872No Hit
GGTCCAGATATACATTGGACGAAGGAAGAGGACGGTCAAAATCACGACAA11.5873015873015872No Hit
AATTATAGCATACAATGGATACACACTCAAGATGCCAAGGCAAATCCTTT11.5873015873015872No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT11.5873015873015872No Hit
CGGCAATATTCACCTGCGGAAGGCTAATATTCGTAACATCTGTCGTGAAG11.5873015873015872No Hit
ATTCAGCGAGTTGGTGTAGAAATTACTGCTATTGACGATATTCACTGAGG11.5873015873015872No Hit
ACTTAATTTCCGTAACGATGTGAATACCACAAGTCATTTTCTTTTCAAAA11.5873015873015872No Hit
CTCTTAAAGTGGAAGAAATTTCTCCCGAAAACCTCCCCGAATATTTGTAG11.5873015873015872No Hit
ATATTCACCTGCGGAAGGCTAATATTCGTAACATCTGTCGTGAAGTTTTG11.5873015873015872No Hit
GGAGTCCCAGCGTCATCCTCCTCCTGGTTACCTTTTGTCTTAAGAATAAA11.5873015873015872No Hit
GTGTGTATCCATTGTATGCTATAATTATTCACTTTAGAATAGTCATCAAG11.5873015873015872No Hit
GTGTTCAAATCGACACTCCAGAAGAATACCAAGGGTCTGTTATCCAAAGC11.5873015873015872No Hit
CAGCGATACAAAGGGTAGTTGGGCTGTGAGGCCTGAGAGTAATTATAGAA11.5873015873015872No Hit
ATTTTGTGCTTTTTACATATTTTGGACATTTTCCAATTGTGATCGGATGT11.5873015873015872No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTGT50.0145.01
GCCCAGA50.0145.0145
CCTTGTG50.0145.02
TGGACGT50.0145.09
CTTGTGT50.0145.03
TGTGGAC50.0145.07
TTGTGTG50.0145.04
GTGGACG50.0145.08
GTGTGGA50.0145.06
TGTGTGG50.0145.05
Produced by FastQC (version 0.11.5)