FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8471308
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG84750.081.879442
CGAAAGC86050.080.6433943
AGCGAAA86150.079.713281
GAGCAAA189250.059.1242941
GAGCGAA37450.051.5069431
CAAAAGC258550.043.0778924
GCAAAAG262350.042.4815673
AGCAAAA287850.038.7429542
ACGGACG10950.035.090744145
AAAAGCA355350.031.9961325
AAGCAGG391950.031.6301637
AAAGCAG423000.030.8731616
GAAAGCA240700.030.065074
AGCAGGG262700.022.381897
ATGCCGG31000.022.217375145
CTCCGAC13000.020.076591145
AGCAGGC133950.019.755538
TACCCCG11050.017.0615635
CACGAGC39700.016.435497145
AGACGGC76000.016.026052145
Produced by FastQC (version 0.11.5)