FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8670085
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA138600.15986002444036015No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT115970.13375878091160584No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA104060.12002189136554026No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA103310.11915684794324394No Hit
ATTCTACACTGTAGAGACCCATTAGAACACATCCAGAAACTGATTGCCCC102700.11845327929310959No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT88180.10170603863745281No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG106500.078.572052
GAGCGAA35000.075.627861
AGCGAAA113200.072.968311
CGAAAGC115000.072.954553
GAGCAAA202200.065.454381
AGCAAAA307200.042.9833872
CAAAAGC325450.040.8185964
GCAAAAG329050.040.3720173
TAGAAAC117950.038.9150542
GAAAGCA237800.035.9211584
GTAGAAA131450.035.6944471
AAGCAGG424700.035.251867
AGTAGAA124100.033.3673131
AAAAGCA414050.032.1191025
AAAGCAG526000.030.3288766
CTACACT66600.029.397234
AGCAGGG219500.029.2317968
CTATACT43300.028.134444
GGGTGTT113300.024.8282477
ACAAGGG165600.023.382873