FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAGTGAGAACTCCATACTTACAAAGTTCACCACATCAGTATCATTTCT23.7037037037037033No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC11.8518518518518516No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACAAACACTACGTCCCCTTGC11.8518518518518516No Hit
GTCTACCTGAAAGCTTGACATGGTGTTGGAGTCCATTATGTTTTTGTCAC11.8518518518518516No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCACTTTCAAAGTCATGCCCACTT11.8518518518518516No Hit
GACACTCAGAATGACAATTGCATCTGTACCTACTTCGCGCTACCTTTCTG11.8518518518518516No Hit
GAACACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGG11.8518518518518516No Hit
GCTATAAACACCAGCCTCCCATTTCAGAATATACATCCGATCACAATTGG11.8518518518518516No Hit
GAATGCTCCTGTTATCCTGATTCTAGTGAAATCACATGTGTGTGCAGGGA11.8518518518518516No Hit
AAATAAGACCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGT11.8518518518518516No Hit
GATCTGGTATTATCATTTCAGATACACCAGTCCACGATTGCAATACAACT11.8518518518518516No Hit
AAACAAACGATGCTGGATCAGACCGAGTGATGGTATCACCTCTGGCCGTA11.8518518518518516No Hit
GTAATCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC11.8518518518518516No Hit
TGGTACTCACTGGTCGGGATAGACCCATTCAAATTACTCCAAAACAGCCA11.8518518518518516No Hit
CATACAACAAGGCAACCAAACGACTTACAGTTCTTGGAAAGGATGCAGGT11.8518518518518516No Hit
AGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTC11.8518518518518516No Hit
CCCAATGATATTTGCTGCAATGACGAGAGGATCACTTGAATCGCTGCATC11.8518518518518516No Hit
ATATTCTTCCCTCATGGACTCAGGCACTCCTTCCGTAGAAGGCCATCTTT11.8518518518518516No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT11.8518518518518516No Hit
ATTTGCAGACAATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCC11.8518518518518516No Hit
GCCCAACCCTTGGGACCAACTCCAGCCCCAGGATGCGACGAGCCGACATC11.8518518518518516No Hit
AGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACTC11.8518518518518516No Hit
GCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGGCCTAC11.8518518518518516No Hit
GATTGCTTCAAATGAGAATGTGGAAACCATGGACTCCAATACCCTGGAAC11.8518518518518516No Hit
GCTTTATTGAGAAGTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAA11.8518518518518516No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG11.8518518518518516No Hit
TCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGACAGA11.8518518518518516No Hit
AACATCCACAGCCCTCTGCTGTTCCTGTTGATATTCTTCCCTCATGGACT11.8518518518518516No Hit
GCTTTCGCTCCCCCCTTCAATGAAACCGGCAATGGCCCCAAATAGGCCTC11.8518518518518516No Hit
CAACTGATCCCCTCAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCT11.8518518518518516No Hit
TCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGCACC11.8518518518518516No Hit
ATCGGGTTCGTTGCCTTTTCGTCCGAGAGCTCGAAGACTCCCCGCCCCTG11.8518518518518516No Hit
GCTAGGATGAGTCCCAATAGTTCTCATTGCATGTACCATCTGCCTAGTCT11.8518518518518516No Hit
ATATAGGACTCCTTTTTGCTCATGTTGATTCCCACTAACTTGCAGGTCCT11.8518518518518516No Hit
GATGATGATTATCGGGGAAGACTTTGTAATCCCCTGAATCCCTTTGTCAG11.8518518518518516No Hit
GAGCATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAG11.8518518518518516No Hit
ATTCATATCATGCTCCCCCTTGGAATGCAGAACCTTCTTCTTGACTCAAG11.8518518518518516No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTTGTGGTAGTAG11.8518518518518516No Hit
GAATGTGCATGTGTAAATGGTTCTTGCTTTACTGTAATGACCGATGGACC11.8518518518518516No Hit
CCATTGCATTTGGGTAAATGTAACATTGCTGGCTGGATCCTGGGAAATCC11.8518518518518516No Hit
ATTCTACACTGTAGAGACCCATTAGAACACATCCAGAAACTGATTGCCCC11.8518518518518516No Hit
AAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTCTACTTCAAGCAGT11.8518518518518516No Hit
CCGTAATATTACAAATTGGAAACATAATCTCAATATGGATTAGACACTCA11.8518518518518516No Hit
ACCTGCATCCTTTCCAAGAACTGTAAGTCGTTTGGTTGCCTTGTTGTAAT11.8518518518518516No Hit
TTGCTGCAATGACGAGAGGATCACTTGAATCGCTGCATCTGCACTCCCAT11.8518518518518516No Hit
GGATGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCT11.8518518518518516No Hit
ATGTATCCTATCTGATATTCCAGATTCTGGTTGAAAGACACCCACGGTCG11.8518518518518516No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA11.8518518518518516No Hit
TTTCTGACATGACCCTCGAGGAAATGTCACGAGACTGGTTCATGCTCATG11.8518518518518516No Hit
AGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGC11.8518518518518516No Hit
CCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGA11.8518518518518516No Hit
GGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAGGATCACTTGAA11.8518518518518516No Hit
GCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAGGAG11.8518518518518516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATG50.0145.09
ACCACTA50.0145.05
CTCTTAT50.0145.0145
ACACCAC50.0145.03
CCACTAT50.0145.06
CACCACT50.0145.04
GAACACC50.0145.01
CACTATA50.0145.07
ACTATAT50.0145.08
AACACCA50.0145.02
Produced by FastQC (version 0.11.5)