FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2075612
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATAACACTGAAATTCGCTT32320.15571311015738973No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC31490.1517142895685706No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG31090.14978714711612767No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC26700.1286367587005664No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA22950.1105697982089138No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAACTACGGAATCTGATGTCGCA21930.1056555849551843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA14950.077.598671
GAGCAAA67150.076.4473951
AGCGAAA24300.066.5386661
GCAAAAG77050.065.120743
CGAAAGC26450.063.3245663
CAAAAGC80000.062.9006924
AGCAAAA80550.061.928122
GCGAAAG27600.060.4204182
AAAAGCA103500.048.8991135
AAGCAGG130500.045.8361787
AAAGCAG141650.043.3051646
AGCAGGG71100.041.8099148
GGATACC7950.041.0422636
AGTAGAA28400.040.082681
TCGCTCC6950.039.644665
TAGAAAC41650.038.994022
CGCTCCC7800.035.324416
GCTTTCG8600.034.5669361
CATACCG1905.4296834E-934.344249
AGCGTCA1956.9921953E-933.465233
Produced by FastQC (version 0.11.5)