FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAATGTCAAATCACCAAGTGAGCATGAAAATGTACACAAACCGAGCGC30.25210084033613445No Hit
GTATGAGGTCACACTTTCAGATTTGTTGCTTTCCCTCAAGGTAGCGCAGC20.16806722689075632No Hit
ATAATAAATAGCGAATAAGTTGAATCAGAGGTCCGGGAAAAGCCAGGGAA20.16806722689075632No Hit
CCACTGCATAGTCTTGGGAATTTGTGGGAGATTATGTCAAAGTATCGCGG20.16806722689075632No Hit
ACTCAAGAATGCTTCGATTGAAGTCTTTTCCTTTTTTTCAGATTACTTAA20.16806722689075632No Hit
GTATACTAGAGGTAATATGATAATTAACAAATTTAGAAAATGGCAAGATG20.16806722689075632No Hit
GTGTAGATAGATTTGTCAGTGAATCCATGCAAACGATGTTCATAATGTAC20.16806722689075632No Hit
ATTAAAAACACCTTGCATTTGTAATCATCATATGGTTTGAAGTCAATTTC20.16806722689075632No Hit
GTCAAAACCTACCGGGCTAGGTGTAACATGGATAGGGCGATCACTTAGGG20.16806722689075632No Hit
ATATTACGCTGGTCATCAGCCGAGTTTACGTCCATACTGCGCCCGAATTA20.16806722689075632No Hit
GTAAATTGGTGTCCAATTGCTTAGCAAATATCCACTATATTTTACTGTGA20.16806722689075632No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)