FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCTGCACTCCAGCCTAGGTGACAAGAGTGAAACACCATGTCAAAAA27.142857142857142No Hit
TAGTAGAAACAAGGGCACTAAAGTATGCAGTAATTCTTTGACATTCAACA13.571428571428571No Hit
CACACACAGACACACACACACAGAGGTACATATGTACATGCCATGCGGAG13.571428571428571No Hit
CATCATAAGGGTAACAGTTGCTGTAGGCTTTGCTGCGTTCAACAAAAAGG13.571428571428571No Hit
GCATCAGAGAGCACATTCTGGGATCCATTCCAGTTCGAACAAGAGCTCTT13.571428571428571No Hit
ATCCAAATGTGCACTGAACTTAAACTCAGTGATCATGAAGGGAGGTTGAT13.571428571428571No Hit
GTGGAAATCTATCCTCAAGGAAATCCCTTCTCAGTGTTCCATCTGCTATT13.571428571428571No Hit
CTTGAACCCAGGAGGCAGAAATTGCAGTGAGCCAAGATTGTACCACTGCA13.571428571428571No Hit
AGGAGGAGAGAAAGGAGAGAGGGGATGGAGAGGAGGAGGAGGAGAAGAAT13.571428571428571No Hit
GAGCGAAGCAGGCAATGAGAGCCATTGGGACTCATCCTAGTTCCAGTACT13.571428571428571No Hit
GCAAAAGCAGGGAGGGATGCAGTCCCGAGGGAGATACCCAGCGCTAGCTT13.571428571428571No Hit
CAGGGGTAGGGAATGGGGAGATAATGCTTAATGGGTGCAGGGTTTTAGGT13.571428571428571No Hit
GTGCTGGACATGCAGATGAATCGTAATTACTGCAGCCAATTAAGCGAATG13.571428571428571No Hit
CTTCTTCAATTAGCCATCTTATTTCTTCAAACTTTTGACCTAGCTGTTCT13.571428571428571No Hit
GACAAGGGTGGACAAACTAACCCAAGGTCGCCAGACTTATGATTGGACAT13.571428571428571No Hit
TTGCTAGTCAGGCCAGGCAGATGGTGCAGGCAATGAGAGCCATTGGGACT13.571428571428571No Hit
CCCCCATTCGTGTCTGATAGGTCTGCAAATTTTCAAGAAGCTCATATCTT13.571428571428571No Hit
ATATTGTGTAGAATTAGACCTTCGTGGGCTTTGGAGCTGTGTTTGAATCC13.571428571428571No Hit
CTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCTCTGGAG13.571428571428571No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT13.571428571428571No Hit
ACTAGAGACCATAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTG13.571428571428571No Hit
GCTACTCGGGAGGCTGAGGCAGGAGAATTGCTTGAACCTGGGAGGCAGAG13.571428571428571No Hit
GAAGTACACATCGGGGAGACAGGAAAAGAACCCGTCACTTAGGATGAAAT13.571428571428571No Hit
GGAAGCATTCTCTTTGAAAACCGGTACAAGATAAGGACACCGTCTCTCAC13.571428571428571No Hit
ATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTTGACATCCTCAATA13.571428571428571No Hit
ATATTATTCAACATCCCTTCATGATAAAAATTCTTCAAAAACTGGGGACA13.571428571428571No Hit
TCAAAGCAGCCACCCATGTTGAAAGCAAATTGTAGAAAAAATTCTGAAAG13.571428571428571No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)