FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4292913
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG53050.078.729282
GAGCGAA23100.078.478831
CGAAAGC53500.078.340883
AGCGAAA58600.070.410781
GAGCAAA114000.067.489321
CAAAAGC153200.049.8880164
AGCAAAA160150.047.540262
GCAAAAG162900.046.739343
AAAAGCA193700.040.093995
AAGCAGG225300.039.678997
AAAGCAG258950.036.5998346
GAAAGCA124850.034.7317774
AGCAGGG148250.029.734638
CTTTCGC15450.024.8740562
GCTTTCG15450.024.4061531
TAGAAAC57900.023.042972
GCAGGGT58600.022.7655939
TCGCTCC17100.022.4749765
TTCGCTC17350.022.150874
AGCAGGT69400.021.729898
Produced by FastQC (version 0.11.5)