FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7685845
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC93400.088.975543
AGCGAAA94500.087.4009861
GCGAAAG110600.075.070952
GAAAGCA213950.039.9947134
AAGCAGG288950.035.762276
AAAGCAG325650.035.38395
GAGCGAA30350.032.0162541
GGTGCGT26450.028.5072828
AGGTGCG28950.027.5479587
AGCAGGG202100.027.0489467
TACGTGC12750.026.15737
GTGCGTT29500.025.5600839
GCAGGGC66350.023.2748458
ATGCCGG36150.022.060799145
CGTAGAC21450.020.2841533
GTATATA114050.018.629311
GAGCAAA150300.018.4301471
CAGGGCG19400.018.31249
ATACGTG18700.018.2259066
ACGTGCC18950.017.981958
Produced by FastQC (version 0.11.5)