FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4408075
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA124500.28243621081764714No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG112280.25471435944261384No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT97030.22011875932237993No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC93140.21129404558679243No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC78480.17803689819252166No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT62260.1412407910482467No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC61340.13915371222132109No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA59350.13463926997612335No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA58060.13171282249054292No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC55190.12520204397611204No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC54800.12431730403861097No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA52910.12002971818764427No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA48740.11056980654820983No Hit
GCGTGAATACAAACCCCAAAATCCCCTTAGTCAGAGGTGACAGAATTGGT47900.10866421283666906No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT46340.1051252530866648No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC46200.10480765413474136No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT45130.1023802907164692No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA45040.10217611996166126No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA34700.087.5562741
GCAAAAG164100.078.7844543
GAGCAAA198200.066.474331
CAAAAGC201600.064.669114
AGCAAAA209200.061.9731562
AGCGAAA53450.056.977621
CGAAAGC57700.055.1679043
AGTAGAA56050.054.3345871
GCGAAAG57700.054.2882352
AAGCAGG295500.053.0686077
AGGCTCG5100.051.175964145
AAAAGCA263950.049.6128165
CAGTACG19650.048.702369
AGCAGGG142700.048.519698
AAAGCAG334100.047.3995176
GCAGGGT71700.046.9177869
GCAGGTA87200.046.8922739
TAGAAAC82650.041.058282
AGCAGGC43950.040.9101838
CGAGTCG1651.36788E-939.545067145
Produced by FastQC (version 0.11.5)