FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6316894
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA39850.084.2559741
GAGCAAA190150.073.147641
GCAAAAG229750.059.8119543
AGCGAAA67900.059.595361
AGCAAAA231400.059.102952
CAAAAGC238100.057.7143824
CGAAAGC72800.057.5745743
GCGAAAG76550.054.4695132
AAGCAGG338300.048.4762087
AAAAGCA289300.047.9015925
AAAGCAG376600.045.0773936
GCTTTCG24450.039.7442171
AGCAGGG203250.037.06158
TCGCTCC26650.035.1018685
GTAACGC9450.029.9282151
CTTTCGC32600.029.5845432
ATTTCGG10100.028.7128728
CGCTCCC32150.028.4202126
AGCAGGC104050.026.0595868
TCCCGTC51850.025.7335622
Produced by FastQC (version 0.11.5)