FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTTTGAGAACAGCCTGACCAACATGCTAAAACCCCGTCTCTACTAAG23.8461538461538463No Hit
AGATGGCACAGCATCATTGAGCCCTGGAATGATGATGGGCATGTTCAACA11.9230769230769231No Hit
GTATGGAAGATATCTTCGTCGGTGAAACCATCACTCCTACAGATGCAGTT11.9230769230769231No Hit
GGATCCAATCTTTGTACCCTGACTTCAGCTCAACTCCCTTGATCTGAAAC11.9230769230769231No Hit
ATCCACTCCTGTGACCTCCTTTGCTGAATTCACTTGCTTAGCCCACAATC11.9230769230769231No Hit
AGCATGGAATATGATGCCGTTGCAACTACACACTCCTGGATTCCCAAGAG11.9230769230769231No Hit
TCAGTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGC11.9230769230769231No Hit
GAATCGGGACAATGGTGATGGAACTAATCAGAATGGTCAAACGGGGGATC11.9230769230769231No Hit
GTCTCATATTATGTTGCTGAAAAATAGAAGGAAAATTCTGCAGGGTCTGA11.9230769230769231No Hit
GCTCAGAGATGGTTCCAATCCTCTGTGCTATGCTCAGACTGGTCTCTGGA11.9230769230769231No Hit
CCATTTCCCGTTTCTGTTTTGGAGTAAGTGGAGATCCCCCATTCTCATTA11.9230769230769231No Hit
GCAAGCTCCACCTCCTGGGTTCACACCATTCTCCTGCCTCAGCCTCCCGA11.9230769230769231No Hit
GACAAAGAATGGTGTTACATTCCAAAGATGCACACTATGAAGAAAGAACA11.9230769230769231No Hit
GTATCAGAACATAAGAAAAAGGGAAAAACAGAAAATGAGCCCAAATAAAA11.9230769230769231No Hit
GAATAAAAGAACTACGGAATCTGATGTCCCAGTCTCGCACTCGCGAGATA11.9230769230769231No Hit
TTTTACAAGTATGCTTTTACGCCATAGCTCTTACGATTAAATATATGAAA11.9230769230769231No Hit
CTGCCCCTTACCAACCAAATTGTTTTGCCTATCCACCCTGTGGTGCCAAA11.9230769230769231No Hit
GTGTGATGGCTTCCAAAATAAGAAATGGGACCTTTTTGTTGAACGCAGCA11.9230769230769231No Hit
ATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCCATTGA11.9230769230769231No Hit
ATTCAGCATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT11.9230769230769231No Hit
TACTCTCGGTCTAGACATCAAAGCAGCCACCCATGTTGGAAAGCAAATTG11.9230769230769231No Hit
CAATTGCTCCCTCTTCGGTGAAAGCCCTTAGTAATACTATGGTCTCTAGT11.9230769230769231No Hit
TTATCTCTCTCTTAAGTTTCCTATACAGTTTAACTGCTTTGTCCATGTTA11.9230769230769231No Hit
GGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGG11.9230769230769231No Hit
GAGCAAAAGCAGGGCACCAGCTTCCCCTGGTTTTCCACTCACCTCTCCAG11.9230769230769231No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC11.9230769230769231No Hit
GGAGAGAGAATGAATGCCAGCAGGGGAAATATCAGATGCTTATAACATCA11.9230769230769231No Hit
CAATAGTCATGTCAGTTATGTATCGCGAAGCAGGTGTGGAGACCATGGTC11.9230769230769231No Hit
TTTCTGCTTCTTGGCTTCTGTGGAGTGCAACAAATTATGATGGACCAACT11.9230769230769231No Hit
GAAGGAGACACAAGAGTCCCTGTGGGCTGAGGGCAAAGACCTGAGAGAGA11.9230769230769231No Hit
TCGAAGACCTCATATTTTTAGCAAGATCTGCATTGATATTGAGAGGATCA11.9230769230769231No Hit
GTTCACAATGGTGGGGAAAAGAGCAACAGCTATACTAAGAAAAGCAACCA11.9230769230769231No Hit
ATGAATATTTGTAAATACTAGAATATGTGGCTAAAACAGTATTTGTAGAA11.9230769230769231No Hit
GAATGAAAGCAAACAATTAAAAATGAACTCAGGTAAGTGACTGATATCAA11.9230769230769231No Hit
GAGGTATTGAGGATAGGAGAGTATATGGGTTTGGCACCATGGGGTGGATA11.9230769230769231No Hit
GCCAATAGAGTCCTACAGACTTTCCCAATGGAACCTTCTTCCACTCCCTT11.9230769230769231No Hit
TCATGGCAGCATTCACTGGAAATACGGAGGGAAGAACTTCAGACATGAGG11.9230769230769231No Hit
GGCGCCATCTTGGCTCACTGCAACCTCTGCCTCCTCGGCTCAAGTAATTC11.9230769230769231No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCCATAACTATTTTAA11.9230769230769231No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG11.9230769230769231No Hit
GTATATGTGGGATGGACTTGTCACAAAATAAACTAAATTCACTTTAAACA11.9230769230769231No Hit
GAAGTAGTTTGAAAGGGTCTATTCCCACCAAGGAATATCCCTCTTTTTCG11.9230769230769231No Hit
AGAGAACACATTCTGGGATCCATTCCAGTTCGGACAAGAGCTCTTGTCCT11.9230769230769231No Hit
GCAGCGTCAGATGTGTATAAGAGACAGAAGTTAGAGTTTAAAGCCAGGTT11.9230769230769231No Hit
TCATGAAACAGCCTCCCGGGCTAGGATATCCCTGGTTTCTCTGTTCTTGC11.9230769230769231No Hit
CCATTGCTCTTTGTGCAGCTGTTTGAAATTTTCCTTTAAGAATGTTGCAC11.9230769230769231No Hit
GTTATCACACTGGTTACTCCTTTCCAGTAAAGACTGATCCCTCCTCACCT11.9230769230769231No Hit
CATAGTGAGTGGAAGAGACGAACAGTCAATAGCCGAAGCAATAATCGTGG11.9230769230769231No Hit
CAGAAGGGTGTTCCAAATGTTGTGCGGTTCACACATGGCCGGAAGGTGAG11.9230769230769231No Hit
GTTTTTCTCCATGATTGCCTGGTCCATTCTGATGCAAAGAGGTCCTTCCA11.9230769230769231No Hit
GTTGTTGCCACCCTCTGCCTATGAGACAGGTGCTGGGAGTCAGCAATCTG11.9230769230769231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTCC50.0145.08
AGAAGGG50.0145.02
ATAGGAA50.0145.0145
AGGGTGT50.0145.05
TGTTCCA50.0145.09
AAGGGTG50.0145.04
CAGAAGG50.0145.01
GGGTGTT50.0145.06
GGTGTTC50.0145.07
GAAGGGT50.0145.03
Produced by FastQC (version 0.11.5)