FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971976
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA6000.071.308721
GAGCAAA19450.058.5358471
GCGAAAG9000.056.4023782
AGCGAAA9550.052.394731
CGAAAGC9700.052.3321043
TCGCTCC4650.049.9044085
GCTTTCG5100.045.5010761
CTTTCGC5200.044.6260572
CAAAAGC26100.041.9544754
CGCTCCC5400.041.6303256
GCGAAGC700.001055383941.438483
GCAAAAG27350.040.302143
AGCAAAA27750.039.459892
AGTCGGC1303.6987512E-739.0377588
AGTAGAA16200.038.496861
TTCGCTC6300.037.9852754
TTTCGCT6550.037.642593
CTCCGAC1553.4708137E-837.41868145
GCAGTCG1406.5970016E-736.258676
AAAGCAG41600.036.084356
Produced by FastQC (version 0.11.5)