FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5564605
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC215310.3869277334150403No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA182110.3272649181747851No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG170680.30672437666285385No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT160780.28893335645566937No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC151960.2730831748165413No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT113900.20468658602003195No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA107050.19237663769485883No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT101430.1822770888499723No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA96430.17329172510897No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT96270.17300419346925794No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC96150.17278854473947386No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA96120.17273463255702784No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC95620.17183609618292764No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA93850.16865527741861283No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC90240.16216784479760915No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC87950.15805254820423012No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG87480.1572079240125759No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG82610.14845617972883968No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT72680.13061124733920917No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG72100.1295689451452529No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT70890.12739448711993034No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT69890.1255974143717299No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATATCAAAA69490.1248785852724497No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG67050.1204937277668406No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA65110.11700740663533171No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTGCAGTTTGTTGGCTT64300.11555177770928933No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT64290.11553380698180733No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT63690.11445556333288708No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA62010.11143648111591029No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG61790.11104112511130619No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA60970.10956752545778183No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT60830.10931593527303375No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA60190.10816580871418546No Hit
CTTATCTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGT58580.10527252158958272No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG56920.10228938082756996No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA56050.10072592753663558No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC55830.10033057153203148No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA16050.082.226431
AGGCTCG4150.078.61429145
AGTAGAA22500.077.347061
GCAAAAG116650.077.269033
TCACGCG300.001936225272.49985145
GCAGGGT58300.071.631279
CGAAAGC29450.068.943253
GCAGGGG17100.066.989979
CAGCGTC2300.066.205622
AGCGAAA30600.065.16681
AGCGTCA2450.062.154453
CGTCAGA2550.059.717025
ATTGCCG3200.058.90612145
AGGGTGT38700.056.7746666
AGCAGGG99150.053.8919078
CGAGTCG2300.053.586838145
CAAAAGC173250.052.988244
GCGTCAG2900.052.5097924
TAGAAAC50050.052.0108262
GCGAAAG39750.051.4417082
Produced by FastQC (version 0.11.5)