FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5659623
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT92630.16366814538706906No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA86400.15266034504418405No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC76030.13433756983459852No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT75390.13320675246390085No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC72790.12861280689544163No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG68590.12119181790023824No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG66920.11824109132357404No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA65980.11658020331036184No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG61970.10949492572208432No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG61030.10783403770887212No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC59890.10581976926731691No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTACAGCTTGTTGGCTT59800.10566074807456256No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG59120.1044592546181963No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGAA32350.063.431241
CGAAAGC27750.062.449073
AGCGAAA33000.050.9759251
GCAAAAG118450.047.8699233
GAGCGAA21500.045.866071
GCGAAAG40150.043.3412482
AAGCAGG156050.042.280297
CAAAAGC153050.039.2746434
AGGGTGT25300.035.5385676
GAGCAAA166700.035.1452641
CAGTACG16150.034.568349
TAGAAAC69950.034.205972
TAAACTA17400.034.1711435
AGCAGGT80950.032.9600228
GGTAGTT25550.032.066237
CGACTCG1153.1641405E-431.521414145
GTAGAAA78150.031.4529761
GCAGGGT41650.030.637839
AGCAAAA195450.029.9744632
CCAACTC46350.029.566723
Produced by FastQC (version 0.11.5)