FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5236436
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT157720.3011972265105503No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC156050.2980080344723014No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC151810.28991092414764547No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG142200.27155874720897955No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA134050.2559947261839923No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA100150.1912560375033706No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT97120.1854696591345717No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA96670.18461029601049264No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC95420.18222317622138418No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA93890.17930134159951538No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC90830.17345767235577786No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT84820.16198040040974435No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC77660.14830697825773104No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA76610.1463017976348799No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT72590.13862482039310706No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC69920.13352593252357137No Hit
GAGCAAAAGCAGGGAGCTTGAAAAGGAGATGGTATTGATTGCAAACAGGA66240.12649825186443603No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT66060.12615450661480443No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG60840.11618589437548746No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT60410.11536472516803413No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG59930.1144480711690165No Hit
TGATAACACAGTTCGAGTCTCTAAAAATCTACAGAGATTCGCTTGGAGAA59510.11364599891987605No Hit
TCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCAGA59150.1129585084206128No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG57010.10887175934165909No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT54380.10384925930537486No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT53070.10134755776638919No Hit
CTTATCTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGT52440.10014444939267853No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA27300.083.668551
GCAAAAG148450.081.473763
GACGCGG9950.071.412099
AGACGCG10400.068.3201758
GAGCAAA184250.066.9439241
CAAAAGC183450.066.403884
GCGAAAG46950.065.945612
AGCAAAA188200.064.726672
CGAAAGC49550.062.193813
AGCGAAA49000.062.1537861
AGTAGAA55300.060.3180161
GCAGGGT63300.058.3019189
TAGAAAC88600.052.131392
AAAAGCA235950.051.751635
AGGGTGT40650.049.2328076
AGCAGGG140650.049.074238
CAGACGC14850.048.3349087
AAGCAGG302900.046.91477
CGAGTCG2600.044.61513145
AGGCTCG8100.042.96272145
Produced by FastQC (version 0.11.5)