FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4639639
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA117900.072.270141
GCGAAAG51450.069.482862
AGCGAAA51950.067.700361
GCAAAAG156750.054.1714063
CGAAAGC66850.053.9107933
GAGCGAA30150.053.3949851
AGCAAAA159800.052.773882
CAAAAGC171200.049.6414684
AAAAGCA205350.041.7391475
AAAGCAG261400.038.3929336
AAGCAGG248600.038.3200537
AGTAGAA72400.031.250061
AGCAGGG143750.028.294128
GAAAGCA144000.025.9841184
GTAGAAA92100.025.5893081
TAGAAAC94050.024.7495353
AACAAGG130000.023.4272882
AGCAGGC78450.023.1964748
TCGCTCC17150.021.9866965
CGCTCCC18450.020.0444726
Produced by FastQC (version 0.11.5)