FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTTTACACAATTGGGTATGTGAAACCAAATATTATTCCATGCAAGTAA11.6129032258064515No Hit
ATTTCACCCTTACGTTCAGAAAGGCTTTGGATAACAGAACCTTGGTATTC11.6129032258064515No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT11.6129032258064515No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT11.6129032258064515No Hit
ATCAAAAGCAGAGAGTACCATTTTCTCTATTGTCAAGCTGTTCTGGATCA11.6129032258064515No Hit
ATCCAGTGGGTACGACTTCGAAAAAGAGGGATATTCCTTGGTGGGAATAG11.6129032258064515No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTG11.6129032258064515No Hit
GGATATTATTTGGGTGAAAAATTTACTTAAGCTTAGAACCTATTTTACAT11.6129032258064515No Hit
GCAGATCTCAAAAGCACTCAAGCAGCAATCGATCAAATCAATGGGAAGCT11.6129032258064515No Hit
CTACTGAGGATGCAGGGTGTTATGTCTGGGCCAGATTTTGGGATTCATCT11.6129032258064515No Hit
GATAAGCAAGTGCAGAACTAAAGAGGGAAGGCGAAAAACCAATTTATATG11.6129032258064515No Hit
AGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT11.6129032258064515No Hit
TATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAG11.6129032258064515No Hit
ACTGTCCAGGCCATTGTTCTGTCTCTCCCCTCGATTATTTCAAATCTGTG11.6129032258064515No Hit
AAGTGCAAGATCCTTCATTACTCATATCAAAAGAGGGCACGATCGGGTTC11.6129032258064515No Hit
GTACCAACCATCCACCATTCCCTCCCAACCATTTTCTATGAAACCCGCGA11.6129032258064515No Hit
TGGCTGCACATCTTAGCTCAAGGCAATGTATAACTCCCAGCCAGGGCTAA11.6129032258064515No Hit
GGGAAGAACTTCAGACATGAGGGCAGAAATCATAAGAATGATGGAAAGTG11.6129032258064515No Hit
CTATCACTCCGACAGATGCAGTTGAAGCTCTTCCAATCCTACACATCGAT11.6129032258064515No Hit
CTAATAGATGCTCTATTGGGAGCCCCTCAGTGTGATGGCTTCCAAAATAA11.6129032258064515No Hit
GATAATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCAT11.6129032258064515No Hit
GTTTCAGGCATCAAAATTCTGAGGGAAGAGGACAAGCAGCAGATCTCAAA11.6129032258064515No Hit
ATGTTATTTGTTCAAAGCTATTTTCAGTTGTTTTTAATCTGTGTCTCACT11.6129032258064515No Hit
AATAAGAGCTTAGACATTGAACACGATGACCAAAGATGCAGAGAGAGGCA11.6129032258064515No Hit
CTTGCAAGTTGCATGGGCCTCATATACAATAGGATGGGGGCTGTAACCAC11.6129032258064515No Hit
GGGTGAACTCATTCTGTATCCAGCTTGAAAGTGACCTTAATTCAGGTTCG11.6129032258064515No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC11.6129032258064515No Hit
GATCCCAAGAAAACTGGGGGGCCCATATACAGGAGAGTAGATGGAAAATG11.6129032258064515No Hit
CTTGGTCAATAACTCACCATTTGCTGGTAAAGAAGGTAAATGGGTGACTT11.6129032258064515No Hit
AGTCTTTTAGTGGTCTTGTTGTAGTTGAATACAGGAGAATTGCCCCTTAC11.6129032258064515No Hit
GACAGGTAGAAAAGATTCTGAAAGAAGAATCTGATGAGGCACTTAAAATG11.6129032258064515No Hit
GACAAGAGCTCTTGTTCGAACTGGAATGGATCCCAGAATGTGATCTCTGA11.6129032258064515No Hit
TGTTGAACTCATTCTGTATCCAGCTTGAAAGTGACCTTAATTCAGGTTCG11.6129032258064515No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG11.6129032258064515No Hit
CACCAGGCCCTATGTCCAACACTGGGGATTACATTTCAACATAAGATTTG11.6129032258064515No Hit
AGTAGAAACAAGGAACAAAGGTAGCTTTTGCCTCTTTCCTGCATTGCCAA11.6129032258064515No Hit
ATTATATTCAGTATGGAAAGAATAAAAGAAGTACGGAATCTGATGTCGCA11.6129032258064515No Hit
TTTCAGTTGTTTTTAATCTGTGTCTCACTTCTTCAATTAGCCATCTTATC11.6129032258064515No Hit
AAGAAACAATTGAAGAAAAATTTGAAATCACAGGAACTATGCGTAGGCTT11.6129032258064515No Hit
GAGGAAGGTAGGAGGGGGAAGGAGGGGGTAAGAAAGAAAGAAAAAAGGAA11.6129032258064515No Hit
GGGGAATGCAGTGGGAAGCAGTGTCCTGGGAAATGCGTGGATTTTAGAGG11.6129032258064515No Hit
ACCAGAGATCTATTTTAGTGTCCTCAACATATTTCTCAAGGTCCTGAATT11.6129032258064515No Hit
TCATTGACAAGAACTCAGTTGAGTATCCGATCAAAACACGCGCTGGAACA11.6129032258064515No Hit
ATCTTAACTATTTTATAATTTTGAACTACTGCAAGTGAATTTTTTTATTT11.6129032258064515No Hit
GTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCACATAGAGGGCA11.6129032258064515No Hit
GCATCTAATTGCTCCTAGGGGTTACTTCAAAATACGAAGTGGGAAAAGCT11.6129032258064515No Hit
CTATTGAGGAATTGTCAAGAAACTGGTTCATGCTAATGCCCAAGCAGAAA11.6129032258064515No Hit
TTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATAGGA11.6129032258064515No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT11.6129032258064515No Hit
GTCACGTTCAATGCTGGGTATTTGAAGTTTAAGTGGGTCAACCAATTCAA11.6129032258064515No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT11.6129032258064515No Hit
ATGCAACATACCAGAGGACAAGAGCTCTTGTTCGAACTGGAATGGATCCC11.6129032258064515No Hit
CCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGAGA11.6129032258064515No Hit
GTACCATTTTCTCTATTGTCAAGCTGTTCTGGATCAACCGCCCTTCATGA11.6129032258064515No Hit
ATTGTTGGCTTGCCGCCAGATTCGCCTTATTTCTTCTTTGTCATAAAGGA11.6129032258064515No Hit
CTCCACACCTGCTTCGCGATACATAACTGACATGACTATTGAGGAATTGT11.6129032258064515No Hit
GGATACATGTTCGAGAGTAAGAGAATGAAGCTCCGGACACAAATACCTGC11.6129032258064515No Hit
GTTATGTATCGCGAAGCAGGTGTGGAGACCATGGTCATTTTAAGTGCCTC11.6129032258064515No Hit
ATGCACACATCACAAAGCAGTTTATCAAAATTTTCTTTCTACTTTTTATA11.6129032258064515No Hit
GGGTCAACACGAAGGGAAACGTCTGTTTGCAATTCTGCCTGCAAACGTTC11.6129032258064515No Hit
CTTGCCAGTTGCATGGGCCTCATATACAATAGGATGGGGGCTGTAACCAC11.6129032258064515No Hit
GTCCTTGGAACCTTCTCCTCATTTTCAACGTCCTGCAATTCTGACAGTAC11.6129032258064515No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTGG50.0145.02
AACCTTC50.0145.09
CCTTGGA50.0145.03
CTTGGAA50.0145.04
GTCCTTG50.0145.01
GAACCTT50.0145.08
TTGATCG50.0145.0145
TGGAACC50.0145.06
TTGGAAC50.0145.05
GGAACCT50.0145.07
Produced by FastQC (version 0.11.5)