FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6871948
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCACACCATCGATTTCTTTTACGATAACTTCTGGACGAGATACTTG171170.24908512113304698No Hit
CTCCAACAGACGCGGTTGAAGCTCTTCCAATCCTACACATCGATGAGCCA157590.22932362119154567No Hit
GAGCAAAAGCAGGAGAAGCGAATGTGTATCGTTTGGCAGAAGTTTCTGTA152960.22258608476082767No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG139340.2027663771611776No Hit
GAGCAAAAGCAGGTGTGTAGTTAAAACTAATACTCGGTGTCATCATATGT122220.17785349947351176No Hit
AAACAAGGATTTGCAAAAAAGGCTCCTTTTTTGTGGATTTTCCAAGCGGC121150.17629644461803262No Hit
CATTTCACACCATCGATTTCTTTTACAATAACCTCTGGACGAGATACTTG100750.14661053896216908No Hit
GAAATATACATTCCCATACCCACCAGAGGCAATTACTACAGCATGTGCAG99970.14547548962826842No Hit
GTTGAATATCCAATCAAACCACGCGCTGGAACAAGGAAGACCAAACGAGT96310.14014948890765763No Hit
GTGTAGCACAAGGGGTTCCTTTTGGTCGTGAATATGGCGGTCAGTTGGAT93710.13636599112798875No Hit
TTCCCATACCCACCAGAGGCAATTACTACAGCATGTGCAGAATGTCTCTC89330.1299922525607004No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA76270.11098745217513288No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA73000.10622897612147239No Hit
ATTCTAAAGTGAATAATTATAGCATACAATGGATACACACTCAAGATGCC71550.10411894851358014No Hit
ATATAATGGTACAAACGGGACTTAATTTCCGTAACGATGTGAATACCACA69110.10056828136650625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA44450.0101.960521
GAGCAAA224150.093.720261
GACGCGG49350.072.280239
AGACGCG50950.070.294478
CAAAAGC300650.069.581814
AAGCAGG394450.063.079847
AGCAAAA331450.062.96142
GCAAAAG334700.062.5463833
AAAAGCA350850.059.708645
GCGAAAG101900.056.855732
CGAAAGC102150.056.7888033
AAAGCAG450650.056.1078576
TCACACC89450.054.7185445
AGCGAAA106450.053.5427441
TTCACAC93300.052.8491944
AGCAGGT137400.052.1325048
ACCATCG93200.051.8861589
AGCAGGG218200.048.4441388
GCAGGGT80100.048.333739
GGGTCAA117900.046.611576145
Produced by FastQC (version 0.11.5)