FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6807936
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA391070.5744325446067647No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA239660.35203033636038883No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT219270.32207999605166676No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT195980.2878699212213511No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT162810.2391473715381578No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT137140.20144137665218947No Hit
ATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACT124410.1827426109763664No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG114040.1675103878767368No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC112520.16527769943783255No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC106950.15709607140842685No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG106730.15677291913437494No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG103650.15224878729764793No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG100820.14809187395416173No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC98930.14531570214526107No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA97550.14328865606257168No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT94700.1391023652396262No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA94600.1389554778423299No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT94250.13844137195179274No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC89990.132183968826969No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT81540.11977198375542897No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT81340.1194782089608363No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA80760.11862626205651756No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA80020.11753929531652471No Hit
ATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTA79140.116246686220317No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT79040.11609979882302067No Hit
TTTCATTTCTGCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTT78600.1154534942749168No Hit
TCTCTGTATATTTTCAGAGACTCGAACCGTGTTACCATTCCATTCAAGTC78440.11521847443924267No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT77300.11354395811006449No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT74400.10928422358847086No Hit
AATATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTC74080.10881418391712261No Hit
AAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTAATGTTAAATACATA73560.10805036945118168No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT71660.10525950890255137No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA71480.10499511158741798No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC70540.10361437005283246No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA70420.10343810517607686No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC69870.10263022449094703No Hit
GGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAAA68900.10120541673717262No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG51500.088.138682
CGAAAGC54300.083.5974663
GAGCGAA16900.075.087341
AGCGAAA68600.065.9592741
ACAAGGG138800.060.4452933
GTAGAAA104800.059.504881
TAGAAAC104100.059.0669562
AAACAAG214400.053.13331
GGTGTTT143550.053.083258
GTGTTTT154250.049.3550579
GAGCAAA161600.047.250071
AGCGAGC9550.044.030853145
CAATTCG39000.043.688879
GGGTGTT186700.043.4550827
AGGGTGT190550.043.116746
GGTCGTT26500.041.039327
AAGGGTG203450.040.7392965
GCAGGTA43200.040.2805189
AGGTCGT27150.039.7961856
CAAGGGT224600.038.581294
Produced by FastQC (version 0.11.5)