FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6593422
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA82850.078.945141
GCGAAAG86150.077.2654952
CGAAAGC89300.074.2980963
GAGCGAA33500.067.100881
GAGCAAA142900.066.8289951
CAAAAGC201200.047.103774
GCAAAAG210850.045.0167473
AGCAAAA216400.043.928222
AAAAGCA271900.035.4691285
AAAGCAG351400.033.5113836
AAGCAGG336900.032.8399357
GAAAGCA223950.030.5005664
GTATAGG34650.026.1588171
AGCAGGG222450.024.281348
TACCCCG28350.023.5311325
CCGCTCA31300.021.5420679
GTAACGC9850.020.612621
GCTTTCG22850.019.9924351
TGCGAGC8950.019.441235145
TCGAACG4553.436071E-919.1239644
Produced by FastQC (version 0.11.5)