FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4745110
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTAAATGGTCTGTTTGGTAGCGAACAAGGCAAGAGATTCGTTAAAT70980.14958557335867873No Hit
GTATTGGATGTGTTCAAAAGATACCAAGAAGAATATGACAGGTATTTAAA64860.1366880852077191No Hit
CTCCAAGCCCGCGTAGCCGTGCAGTACGTCGAATTTTGCGCACAGGTAAT57580.12134597511964948No Hit
TTGTAGTAGTGGGTGGCAACCTGGTATTCGTGTGGCTCCCATTCATGATA50660.10676254080516573No Hit
TCGTAAAGGTGGTGCAGGCGGTTGGGAACAGCTTTGATGACCGGCCTTAA50420.1062567569561085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG79400.059.9071662
GAGCAAA99200.054.165761
CGAAAGC98200.049.3263173
GCAAAAG120850.044.161613
GAAAGCA128500.038.485314
AGCAAAA144250.036.9962232
AGCGAAA133200.035.0045971
CAAAAGC154900.034.8285374
AAGCAGG187350.034.718926
AGCAGGG95900.033.719037
AAAGCAG228950.031.7988475
AGCAGGC62450.027.167328
AAAAGCA223800.024.3979425
GCAGGGC49900.022.0854598
ATGTGTT44200.021.652858
ATTGGAT63150.018.1425133
TGGATGT57100.018.0331065
GCAGGGA68000.017.8061879
GGATGTG54900.017.5673716
GAGCGAA84850.017.092141
Produced by FastQC (version 0.11.5)