FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6180781
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA313660.5074763205491345No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG292930.4739368697904035No Hit
TAGTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTT260630.42167810184505805No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC214550.3471244168010483No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC162450.2628308623133549No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA138310.2237743094278862No Hit
GTAATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTC134300.21728645619380466No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTTCTTA128350.20765984104597784No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA121910.19724044582715353No Hit
GAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATTGCTTT121740.1969653996800728No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC118290.1913835808128455No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT117050.18937736185766815No Hit
TAGTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATC116500.18848750667593625No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA110830.17931390871153663No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT110350.17853730782566152No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT105210.17022120667274898No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTATGTG103990.1682473460878164No Hit
GTATAGGAAACTTAAGAGAGAGATAACGTTCCATGGGGCCAAAGAAATAG102740.16622494794751666No Hit
CAAGTTGTAGACCAAGAACTGAGTGATGCCCCATTCCTTGATCGGCTTCG93130.15067675104489223No Hit
ACTATGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCT88540.14325050507371156No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAATGAGAAAGTT88070.14249008337295888No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC87950.14229593315149008No Hit
CATCCTAGTTCCAGTACTGGTTTAAGAGATGATCTTCTTGAAAATTTGCA85930.1390277377567657No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG85730.13870415405431774No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA78730.12737872446863915No Hit
GTATACATCGTGGTCATAAGTTCCATTTCTGATTGATCCTATGCAGGCAT78650.12724929098765997No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG78260.12661830276788646No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTT78000.12619764395470412No Hit
GAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCT77230.12495184670027945No Hit
GTATCAGCAGATCCACTAGCATCTTTATTGGAGATGTGCCACAGCACACA77070.12469297973832111No Hit
TCCTTACTCTGGGTCTAGATCCGATATTCGGGATTGCAGTTTGTTGGCTT76640.12399727477805798No Hit
GCATCCACAGCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTC75800.12263822322777657No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGT75690.12246025219143017No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT75610.12233081871045098No Hit
ATACTATGGTCTCTAGTCGGTCAAAAATCACACTGAAATTCGCTTTCAAC74380.12034077894039605No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA72640.1175256007290988No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG71920.11636069940028615No Hit
GAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAG71530.11572971118051262No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTCTGACTAAGGG71330.11540612747806467No Hit
ATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACAATTGC68470.11077888053305884No Hit
GGGTACACCATGGACACAGTCAACAGAACACATCAATATTCAGAGAGGGG67360.10898299098447267No Hit
GGTATGTGCAACATGTGAGCAGATTGCTGACTCCCAGCACAGGTCTCATA67080.10852997380104554No Hit
GAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAATCATGG66840.10814167335810798No Hit
GAACTGAGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAGGTC65470.10592512499633946No Hit
CTCCTGTTCCACTTCAAACAGCAGTTGTAATGCTTGCATGAATGTTATTT65450.10589276662609466No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT63860.10332027619163338No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTAT63660.10299669248918543No Hit
GAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAA63430.10262457123137027No Hit
GAATCACAGTCTCTACCAAAAGAAGCCAACAAACTGCAATCCCGAATATC63240.10231716671404471No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT62780.1015729241984144No Hit
ATCTGGGACAGGCCCCGTATGTGATCCTGTTTACATTTTGGAATGGTTTG62160.10056981472082573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA34450.0107.5564351
AGGCTCG6900.0106.12318145
GCAAAAG179350.089.672933
GCAGGGT89150.081.8948449
GCAGGGG28800.080.5574959
AGTAGAA29700.077.882331
CAAAAGC229300.070.9610444
AGCAGGC11550.067.165578
AGCAGGG146150.066.176228
CGGCTCG1009.094947E-1265.249985145
CGAAAGC57950.063.689164
GCGAAAG58200.063.415583
AGCGAAA60550.060.5926552
AAGCAGG337200.058.826567
GAGCAAA280900.057.8488581
AGGGTGT50450.057.6347436
AGCAAAA291250.055.7655142
TCACGCG400.006052367454.374992145
GCAGGTA140200.052.9024939
TAGAAAC71400.051.994252
Produced by FastQC (version 0.11.5)