FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4954013
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG355740.718084510476658No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA202690.4091430523093097No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT166490.3360709792243177No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG123860.250019529621743No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC111550.22517098764173613No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA111320.22470671756412425No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT96170.19412544940838872No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA94360.1904718457541391No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA93680.189099221176852No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT89690.1810451446130642No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG89180.18001567618009884No Hit
GAGCAAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAAAGCACACAGAA88510.17866323725836006No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC86450.17450499221540194No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC85680.17295069673817973No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGAAAACAAATCGTG83760.1690750508728984No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA79550.16057688988704713No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT77270.15597456042202554No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT76480.15437989363370666No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT76330.15407710880048156No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC72790.14693138673636907No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA72090.14551839084798526No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT69560.14041141999425516No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT64080.12934968075376466No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT63910.1290065246094429No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC60350.12182043123423374No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA59450.1200037222348831No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG55950.112938742792964No Hit
GTACTATGTTCTTTTCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGG55460.11194964567109533No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT54880.11077887764929159No Hit
GGAATAGGCAATTCTCCAGTATTCAATTACAACAAGGCAACCAAACGACT53880.10876031209445756No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCT52350.10567190679556149No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC52270.10551042155117478No Hit
GACACATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGA52140.10524800802904635No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT51990.10494522319582124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA32700.0132.163791
AGGCTCG5050.0100.493355145
GAGCAAA209600.087.492641
GCAGGTA58850.082.9127359
GCAGGGT121750.080.2141959
AAGCAGG310100.073.412797
AGTAGAA34900.071.8893661
GCGAAAG63400.068.280183
AGCAGGG182650.067.36068
CGAAAGC64300.067.324474
GCGTCAG2300.066.207241
CAAAAGC290650.063.7925574
GCAAAAG295600.062.4544753
GTAGAAA50700.059.3545651
AGCAAAA315300.058.206172
CGGCGGC1750.057.99902145
CGTCAGA2800.056.9725272
AAAGCAG418500.054.7180526
AGCAGGT124000.053.732588
ATAGGCA16550.052.5766454
Produced by FastQC (version 0.11.5)