FastQCFastQC Report
Fri 21 Sep 2018
HMLHWBGX7_n01_HK01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLHWBGX7_n01_HK01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCATATTCGGGTTGTCCCAGCGGTGTGTCCAGTAATAGGTTTTGACGG12.0408163265306123No Hit
CCTATATAGAGATATATAGATATAGAGAGTTCTTCATTTGGATTCTAAGA12.0408163265306123No Hit
GAAGGAAGGAAGGAGGAAGAAAGAAGAGGAGAGGAGAGGATATTACGTAT12.0408163265306123No Hit
CAATTACCTAATTATCTAACAAAGTCTATAATCTAAATTAGAAACACAGT12.0408163265306123No Hit
GTGTAGTTAAAACTAATACTCGGTGTCATCATATGTCGTATAGCCTTCAC12.0408163265306123No Hit
TTATGAAACAGATTAGAATTATTATTGAGCGCAGCAAGGATTTTTATTGG12.0408163265306123No Hit
CCATGAAACTCATCATCCTCGACCGCGACGGCGTCATCAATCAAGACCGC12.0408163265306123No Hit
TGGGTGGATTACCTGTGCGCAAAATTCGACGTACTGCACGGCTACGCGGG12.0408163265306123No Hit
GGGGCAAGCAGGGTCGGCTCGCCCGGCTGCAATACTGCGCCGTGCAGCAT12.0408163265306123No Hit
TACCAGTTGTCTCGCCAGAGGCATCGCTGGGTAGCTATGTAGGGAAGGGA12.0408163265306123No Hit
CAGTTGGTCGGCTTTGTCGATTTGATAACTGCTTAAAAGATAGTAAGCCG12.0408163265306123No Hit
GGCTTATTACTTTGAGTCTAAAGTGATGAGTGCAACTTCAACATCTTGTG12.0408163265306123No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA12.0408163265306123No Hit
GAGCAAAAGCAGGTGCTACTGCAATTATAACTTCATCACTTCTAGTTCCT12.0408163265306123No Hit
ACACCATTCAAGCCAAGCTCTTACACAGCAACAGCCGCAACCTCCATCTG12.0408163265306123No Hit
GGCTTACACTCGTCCCAAAAGTGGTATATCCTGAGATATGTCTATTTGGA12.0408163265306123No Hit
CAGACGTTTCCCTTCGTGTTGACCCAACTGATTCACCAGATAAATGGACT12.0408163265306123No Hit
GTATAGCCTTCACAAAATATTTATTTCCAAAATTTTTGTCTCCATAGAGT12.0408163265306123No Hit
GAATACACAAACCCAGTGGCAACAAAAGGAACGCAAGAGTCAGTACGCGT12.0408163265306123No Hit
ATAAAGGACTGGTTATTGATTTTTAGAGGCATAGCCTTACTTAGATAGAC12.0408163265306123No Hit
GTTCCATCTACTGTGCCGGAGGAAATATGGAGGCTACTCGCCTTTCCGGA12.0408163265306123No Hit
GTGTGTCCATCCATACCCAGGCATCCCTCAGCCCTGGCAAGGAAGGCGGT12.0408163265306123No Hit
CAAGAGTCCCTGTGGGCTGAGGGCAAAGACCTGAGAGCGACCAGGGAGGC12.0408163265306123No Hit
CCCTGGTTCTTGTGTTCCTTTTGTCGCTACTGGATTAGTATATTCTGGAG12.0408163265306123No Hit
ACAGAAAGCTTTAATAGATATATATTTTCATCTAAGTTTTCTATATTAGT12.0408163265306123No Hit
GTTCTGAGCGAAAAGGGGATTATGCCAGTGATTACTCCTGTGAAACCCTA12.0408163265306123No Hit
TAGTTTGTTCATCACTTTAGTTGGCGTGTTGCTCCATCGGCCCATTCCAC12.0408163265306123No Hit
CTGTTTATACTTGTTAGAAGGGTCTGCCGTTCCAGATTAGGGAGTGAAAT12.0408163265306123No Hit
GGCGAATTATCGAGCCATGCAAAAAAAAATAAAGGAATTTTTTTGTTCAT12.0408163265306123No Hit
AACTGAAGAAGATATAGTTTCAAACTATATCTTTTTCTTTTAATAAAAAA12.0408163265306123No Hit
ATCTTCCACTTTTCCTTGCTTATCTTGCCGCATTTATCCGCCCCTCGGTA12.0408163265306123No Hit
AGTCACAGGTACTTCCGGATAAGTTTTCTTCGACTCTTTCCAAACTTCTA12.0408163265306123No Hit
CTCCAAGCCCGCGTAGCCGTGCAGTACGTCGAATTTTGCGCACAGGTAAT12.0408163265306123No Hit
AATATGAAGGATGGGGTATTGAACCCTGAGGGAAAAGCCGTTAAAACTGC12.0408163265306123No Hit
TTTAAGAAACTTCCTTTTTTTCCAACTGCTAAAACTGTTATAGCATTTGG12.0408163265306123No Hit
AATCCCTATCCATGCGTATCGAACCGCAGGTATGGGCTCGGCTGGCGGCC12.0408163265306123No Hit
GTATTCAATAGCCTGTATGCATCACCACAATTGGAAGGATTTTCAGCGGA12.0408163265306123No Hit
CTACTGATCCTTTGAATTTCTGCAGTACCAGTTTTACTGTGTCATTTTCA12.0408163265306123No Hit
ATAATATACATGACTCCTTCTGCAAAGATCCAGACGAGCGATTGCGTTTC12.0408163265306123No Hit
TAAAAATCCTCGTCAGAATCTATTGTGAAAGAACGGGATTTGAAGTCTTT12.0408163265306123No Hit
CCTTAAAGGAAGAGAGGTCCAACAAAAAGCTGGAAGCTGAAAAATTGTTT12.0408163265306123No Hit
AGTAAGGAGCTTCGTCGTCGAGCAGCTATCGAGCGAGACGGAGGCGAAGT12.0408163265306123No Hit
GCCTTTATGGGGGATTCAGACTATCCAGATCCCATAAAAAGCATCGCTTG12.0408163265306123No Hit
AAAACGCACAGAGCGAACTTCATTTCTGCGATTTGATTTCCCGTATCATG12.0408163265306123No Hit
ACCTTTCACCCCGGTATTAGAAACCCCTTGAATAATATATTTCTGTAAAA12.0408163265306123No Hit
CAGCATCATGGCAAATACCGGATAAAGGGGAATCGGCATTCCGGTAATAG12.0408163265306123No Hit
GGTTTTAGTCTATATAAAATTTTTAAGTACAACAATCCATAAAATAATAT12.0408163265306123No Hit
CCAAATGTTCCGTAATACTCCCCAAATGCAGTCCATTTTTCAACAAAAGA12.0408163265306123No Hit
CTCTTAGGTTTGCTTTACCTGCTTTTATTTCTTCCATTGTAGGAGTATTT12.0408163265306123No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)