FastQCFastQC Report
Fri 12 Feb 2021
HMLGTAFX2_n02_QG2935.mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLGTAFX2_n02_QG2935.mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9939046
Sequences flagged as poor quality0
Sequence length149
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGCCGACGACCACCAGGCAGTGTAG4280764.307012966838065No Hit
GCTGTCTCTTATACACATCTGACGCTGCCGACGACCACCAGGCAGTGTAG100780.10139806174556391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)