FastQCFastQC Report
Fri 12 Feb 2021
HMLGTAFX2_n01_QG2939.mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMLGTAFX2_n01_QG2939.mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18023970
Sequences flagged as poor quality0
Sequence length149
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACGACGAGATTAATCTC2075661.15161088261909No Hit
ACTGTCTCTTATACACATCTCCGAGCCCACGAGACGACGAGATTAATGTC449860.24958985173632667No Hit
TCTGTCTCTTATACACATCTCCGAGCCCACGAGACGACGAGATTAATCTC270540.15010011667795717No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)