FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19069605
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4009072.10233510342768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG4304.783942E-1013.02257859
TACGTAC5004.0745363E-1011.89850752
CCGTATC10100.011.78026816
TACGGAC4802.7066562E-911.66902862
CGTAGTA6301.8189894E-1211.66902866
GTACGAT3104.5616747E-511.3484791
TGCTACG5904.7293724E-1111.27024649
GTCGGTT9750.011.1855281
GTCCATT19000.011.1095651
GTCGCGC2550.001301487411.0369531
CGTACGA5401.4642865E-911.01667554
GCCGTAT9600.010.93589915
CTACGTA5301.2820237E-810.56479651
CGGGATT10600.010.56454735
GCTACGT5653.086825E-910.52995750
CGTTCCG10650.010.51461722
CCCGTAT6057.858034E-1010.4668631
TACGACT6109.913492E-1010.3271153
CCGTATT8150.010.309242
GTACGAA6452.382876E-1010.30835255
Produced by FastQC (version 0.11.5)