FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG036.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG036.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16855786
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG429550.254838308934392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA10950.025.25038170
CGTATCG20900.023.6117317
GTTTATT121450.020.2040581
CGACCGT17050.019.50151635
GTTACGA24650.019.0285631
TCGACCG17550.018.9458634
TTCGGCG27750.018.7933986
TACGACT18750.018.4802113
GTTCATT118450.018.0856631
GTTTAAT76900.018.0254841
CGTTCGA22400.017.81344445
GTTCGCT33350.017.6332171
GTCACAT119250.017.3185581
GTTCGAT39300.017.2793461
GTCCATT92950.016.871231
GTTTGAT130200.016.6417031
CCGTATC31050.016.11891716
GTTTTAT96600.016.0888121
TCGGCGC34650.015.8588627
GTCCGAA19650.015.8542451
Produced by FastQC (version 0.11.5)