FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG035.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG035.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21019185
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG389670.18538777788006527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG25100.025.37760717
CGCGTAA12050.022.65487370
TATGCCG16200.021.38957448
TTCGGCG32400.019.7694346
CCGTATC34800.019.41034116
CGTTCGA26150.018.06954245
GTTTATT141300.017.8361551
GTTCGAT47250.017.1127991
TATCGTT38500.016.63582819
AAGTGCG30750.016.50475140
CGCTACG10000.016.44950959
GCCGTAT40200.016.36798315
GTCACAT149850.015.8607571
TCGGCGC42450.015.8310097
CGTTCCG41000.015.7069622
GTTCGCT37750.015.3922471
GTCCATT110800.015.3219081
GCGTTCG31000.015.24264144
GTTTAAT94200.015.1978881
CGGCGCT45000.015.1672618
Produced by FastQC (version 0.11.5)