FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG034.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG034.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15244170
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG254720.16709338717686828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA11450.031.78930770
TATGCCG12750.027.72645246
CGTATCG32000.027.23344217
TACGACT18650.025.8991073
CGCTACG9800.024.99939259
CGACCGT17900.024.83236735
TTCGGCG37150.023.836946
CGTTCGA27050.023.03214645
ACGGCTA15700.022.96114732
CCGTATC39550.022.30019616
GTTACGA26250.021.8685721
TCGGCGC42400.021.3807377
GCCGTAT41900.021.21722415
TCGACCG21450.021.21160534
GTTTATT106900.020.8577581
CGGCGCT43200.020.8228238
GTTCGCT28100.020.8025251
AAGTGCG29950.020.80239940
AGTGTCG30700.020.5210152
TGCCGTA44550.020.19110114
Produced by FastQC (version 0.11.5)