FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG033.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG033.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18188443
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG238680.13122618577082162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG31850.030.76831617
CGCGTAA10850.025.16054270
TTCGGCG40350.024.3755826
CCGTATC43150.023.35974316
TCGGCGC46400.022.4038937
TATCGTT46050.021.66059519
GCCGTAT46400.021.4221315
CGGCGCT49450.021.0927188
GTTTATT126050.019.6888621
CGTTCCG51600.019.53435722
TGCCGTA51400.019.47466514
GTTCGAT45750.018.8218041
GTATCGT55300.018.41717518
CGACCGT14700.018.33349435
ATCGTTC55600.018.0660120
GTTCCGC56250.017.79507623
CGGGATT59050.016.89261235
GTTCGCT34000.016.7813131
CGTTTCG17650.016.658019
GTCCATT96850.016.5171011
Produced by FastQC (version 0.11.5)