FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG032.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG032.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19518890
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG273770.14025900038373082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19250.034.54623846
TCGTATG27200.025.60746243
CGTATGC31150.022.24803744
CGCGTAA13400.021.15622170
CGTATCG30350.020.64202117
ACGATCA38250.018.94112232
CGCTACG16900.017.81023459
GTACACT89750.017.6676331
TTCGGCG38950.017.1635076
CGTTTCG21250.017.130249
CTCGTAT39500.017.10178642
GTTTATT119300.016.2548871
ATGCCGT42050.016.23108747
CGTCTGA45450.016.0173116
ACACGTC44800.015.703402513
CGTTACG13650.015.64134148
TCACGAT45250.015.54679730
CGATCAG46600.015.47209833
GTATCGT42200.015.09443318
GTTCGCT36150.015.0085251
Produced by FastQC (version 0.11.5)