FastQCFastQC Report
Wed 3 Jul 2019
HMKYYBGXB_n01_GG031.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMKYYBGXB_n01_GG031.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20176462
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG336100.16658024583299094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11700.021.24016446
CGTATCG17900.017.98820917
CGCGTAA5300.017.82957570
GTCCGTT35850.017.7698961
GTCCGGT25150.016.9794831
GTCCGAA24150.016.0882381
GTCCGCT45100.015.7551351
GTCCATT99250.014.9533311
GTTCGCT35100.014.8587331
GTCCGGA31750.014.772831
GTCCTAT49200.014.4422061
GTCCGCA38000.014.27745151
GTCGTAT22400.013.5948311
GTACACT87500.013.4810721
GTTCGTT28850.013.4672771
GTCGGAT27100.013.4328021
GTCGGTT34500.013.2909131
GTCCAAT75150.013.08820151
GTCGTAA21050.012.6375851
GTCCGTA18850.012.6269991
Produced by FastQC (version 0.11.5)